SIRT2 Direct Fluorescent Screening Assay Kit

Cayman Chemical Item Number 700280

SIRT2 Direct Fluorescent Screening Assay Kit

Description

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s SIRT2 Direct Fluorescent Screening Assay provides a fluorescence-based method for screening SIRT2 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(ε-acetyl)-AMC, is incubated with human recombinant SIRT2 along with its cosubstrate NAD⁺. Deacetylation sensitizes the substrate such that treatment with the Developer in the second step releases a fluorescent product.

Stability

1 year

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

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Dali-Youcef, N., Lagouge, M., Froelich, S., et al. Sirtuins: The 'magnificent seven', function, metabolism and longevity. Ann Med 39 335-345 (2007).

North, B.J., Marshall, B.L., Borra, M.T., et al. The human Sir2 ortholog, SIRT2, is an NAD⁺-dependent tubulin deacetylase. Mol Cell 11 437-444 (2003).

Hiratsuka, M., Inoue, T., Toda, T., et al. Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene. Biochem Biophys Res Commun 309 558-566 (2003).

Imai, S., Armstrong, C.M., Kaeberlein, M., et al. Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase. Nature 403 795-800 (2000).

Dryden, S.C., Nahhas, F.A., Nowak, J.E., et al. Role for human SIRT2 NAD-dependent deacetylase activity in control of mitotic exit in the cell cycle. Mol Cell Biol 23(9) 3173-3185 (2003).

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Tanner, K.G., Landry, J., Sternglanz, R., et al. Silent information regulator 2 family of NAD-dependent histone/protein deacetylases generates a unique product, 1-O-acetyl-ADP-ribose. Proc Natl Acad Sci USA 97(26) 14178-14182 (2000).