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Demethylase (Jumonji-type) Activity Assay Kit

Cayman Chemical Item Number 700390

Demethylase (Jumonji-type) Activity Assay Kit

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Description

Lysine demethylases containing Jumonji C (JmjC) domains produce formaldehyde following 2-oxoglutarate-dependent demethylation. Cayman’s Demethylase (Jumonji-type) Activity Assay provides a convenient fluorescence-based method for assaying JmjC-mediated demethylase activity from cell lysates or purified enzyme preparations. The assay is based on the production of formaldehyde during the demethylation of a methylated peptide substrate. Cyclization of formaldehyde and acetoacetanilide in the presence of ammonia gives a fluorescent product for quantitation.
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Schneider, J., and Shilatifard, A. Histone demethylation by hydroxylation: Chemistry in action. ACS Chem Biol 1(2) 75-81 (2006).

Katoh, M., and Katoh, M. Identification and characterization of JMJD2 family genes in silico. Int J Oncol 24 1623-1628 (2004).

Rose, N.R., Woon, E.C.Y., Kingham, G.L., et al. Selective inhibitors of the JMJD2 histone demethylases: Combined nondenaturing mass spectrometric screening and crystallographic approaches. J Med Chem 53 1810-1818 (2010).

Strahl, B.D., and Allis, D. The language of covalent histone modifications. Nature 403 41-45 (2000 Jan 6).

Rose, N.R., Ng, S.S., Mecinovic, J., et al. Inhibitor scaffolds for 2-oxoglutarate-dependent histone lysine demethylases. J Med Chem 51 7053-7056 (2008).

Li, Q., Sritharathikhun, P., and Motomizu, S. Development of novel reagent for Hantzsch reaction for the determination of formaldehyde by spectrophotometry and fluorometry. Anal Sci 23 413-417 (2007).

Tian, X., and Fang, J. Current perspectives on histone demethylases. Acta Biochim Biophys Sin 39(2) 81-88 (2007).

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700390-96well
96-Well Cover Sheet
Half Volume 96-Well Solid Plate (black)
JMJ Assay Buffer 10 mL
JMJ Ammonium Acetate 5 mL
JMJ Formaldehyde Detector 150 mg
JMJ DMSO 2 mL
JMJ Positive Control 50 µL
JMJ Control Peptide 200 µL
JMJ Formaldehyde Standard 100 µL
Size Price Quantity Subtotal
96 wells $300.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Demethylase (Jumonji-type) Activity Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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