Ascorbate Assay Kit

Ascorbate Assay Kit

Cayman Chemical Item Number 700420

L-Ascorbic Acid Assay Kit; Vitamin C Assay Kit

Description

Ascorbate (L-Ascorbic acid or Vitamin C) is a six-carbon lactone that is synthesized from glucose in the liver of most mammalian species, but not by humans. Therefore, humans must obtain ascorbate in their diet in order to survive. In humans, ascorbate acts as an electron donor for eight different enzymes. It also serves as an antioxidant and may be beneficial for reducing the risk of developing chronic diseases such as cancer, cardiovascular disease, and cataracts. Cayman’s Ascorbate Assay provides a reproducible, sensitive fluorescence-based tool for quantifying ascorbate from plasma, serum, urine, and fruit juices.

Synonyms

L-Ascorbic Acid Assay Kit
Vitamin C Assay Kit

Stability

6 months

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Eipper, B.A., Milgram, S.L., Husten, E.J., et al. Peptidylglycine α-amidating monooxygenase: A multifunctional protein with catalytic, processing, and routing domains. Protein Sci 2 489-497 (1993).

Koshiishi, I., and Imanari, T. Measurement of ascorbate and dehydroascorbate contents in biological fluids. Anal Chem 69(2) 216-220 (1997).

Vislisel, J.M., Schafer, F.Q., and Buettner, G.R. A simple and sensitive assay for ascorbate using a plate reader. Anal Biochem 365(1) 31-39 (2007).

Schorah, C.J., Downing, C., Piripitsi, A., et al. Total vitamin C, ascorbic acid, and dehydroascorbic acid concentrations in plasma of critically ill patients. Am J Clin Nutr 63(5) 760-765 (1996).

Diliberto, E.J., Daniels, A.J., and Viveros, O.H. Multicompartmental secretion of ascorbate and its dual role in dopamine β-hydroxylation. Am J Clin Nutr 54(6) 1163S-1172S (1991).

Peterkofsky, B. Ascorbate requirement for hydroxylation and secretion of procollagen: Relationship to inhibition of collagen synthesis in scurvy. Am J Clin Nutr 54(6) 1135S-1140S (1991).

Gropper, S.S., Smith, J.L., and Groff, J.L. The water-soluble vitamins. 4 259-275 (2005).

Padayatty, S.J., Katz, A., Wang, Y., et al. Vitamin C as an antioxidant: Evaluation of its role in disease prevention. J Am Coll Nutr 22(1) 18-35 (2003).

Rebouche, C.J. Ascorbic acid and carnitine biosynthesis. Am J Clin Nutr 54(6) 1147S-1152S (1991).

Englard, S., and Seifter, S. The biochemical functions of ascorbic acid. Annu Rev Nutr 6 365-406 (1986).

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700420-96well

96-Well Cover Sheet