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703002 Glutathione Assay Kit
GSH Assay Kit
Glutathione Assay Kit Workbook Links
Cayman offers a variety of analysis tools to assist you.
Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Cayman’s GSH assay kit utilizes a carefully optimized enzymatic recycling method, using glutathione reductase for the quantification of GSH.1,2,3 The sulfhydryl group of GSH reacts with DTNB (5,5'-dithio-bis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by glutathione reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to this recycling reaction which in turn is directly proportional to the concentration of GSH in the sample. Measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of GSH in the sample. GSH is easily oxidized to the disulfide dimer GSSG. Because of the use of glutathione reductase in the Cayman GSH assay kit, both GSH and GSSG are measured and the assay reflects total glutathione. The kit can also be used to measure only GSSG by following an alternative protocol. GSH measurement can be done in plasma, tissue samples, and cultured cells using this kit. Nearly all samples require deproteination before assay.
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1
Baker, M.A., Cerniglia, G.J., Zaman, A. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190 360-365 (1990).
2
Eyer, P., Podhradsky, D. Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman’s reagent. Anal Biochem 153 57-66 (1986).
3
Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 27 502-522 (1969).
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Purchase 703002 Glutathione Assay Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
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Pricing updated 2010-03-19.
Prices are subject to change without notice.
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