See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Glutathione Assay Kit

Cayman Chemical Item Number 703002

GSH Assay Kit

Glutathione Assay Kit

See analysis tools…

Glutathione Assay Kit Analysis Tools

Cayman offers a variety of analysis tools to assist you.

See more

Description

Cayman’s GSH assay kit utilizes a carefully optimized enzymatic recycling method, using glutathione reductase for the quantification of GSH.1,2,3 The sulfhydryl group of GSH reacts with DTNB (5,5'-dithio-bis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by glutathione reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to this recycling reaction which in turn is directly proportional to the concentration of GSH in the sample. Measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of GSH in the sample. GSH is easily oxidized to the disulfide dimer GSSG. Because of the use of glutathione reductase in the Cayman GSH assay kit, both GSH and GSSG are measured and the assay reflects total glutathione. The kit can also be used to measure only GSSG by following an alternative protocol. GSH measurement can be done in plasma, tissue samples, and cultured cells using this kit. Nearly all samples require deproteination before assay.

1 Baker, M.A., Cerniglia, G.J., and Zaman, A. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190 360-365 (1990).

2 Eyer, P., and Podhradsky, D. Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman’s reagent. Anal Biochem 153 57-66 (1986).

3 Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 27 502-522 (1969).

Synonyms
  • GSH Assay Kit
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Eyer, P., and Podhradsky, D. Evaluation of the micromethod for determination of glutathione using enzymatic cycling and Ellman’s reagent. Anal Biochem 153 57-66 (1986).

Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 27 502-522 (1969).

Baker, M.A., Cerniglia, G.J., and Zaman, A. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190 360-365 (1990).

703002-96WELL
96-Well Solid Plate (Colorimetric Assay)
GSH MES Buffer (2X) 1 ea
GSH DTNB 4 × 1 ea
GSSG Standard 1 ea
GSH Co-Factor Mixture 1 ea
GSH Enzyme Mixture 1 ea
703002-480well
96-Well Solid Plate (Colorimetric Assay)
GSH MES Buffer (2X) 1 ea
GSH DTNB 6 × 1 ea
GSSG Standard 2 × 1 ea
GSH Co-Factor Mixture 5 × 1 ea
GSH Enzyme Mixture 5 × 1 ea
Size Price Quantity Subtotal
96 wells $230.00 $0.00
480 wells $430.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glutathione Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

4-hydroxy Hexenal
4-hydroxy Nonenal
8-hydroxy-2-deoxy Guanosine EIA Kit
8-Isoprostane EIA Kit
Aconitase Assay Kit
Aconitase Fluorometric Assay Kit New
Antioxidant Assay Kit
Ascorbate Assay Kit
Calcium Assay Kit
Catalase Assay Kit
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit
Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Glutathione Peroxidase Assay Kit
Glutathione Reductase Assay Kit
Glutathione S-Transferase Assay Kit
Glutathione S-Transferase Polyclonal Antibody
Hydrogen Peroxide (urinary) Assay Kit
iPF2α-VI EIA Kit
Lipid Hydroperoxide (LPO) Assay Kit
L-Theanine Fluorometric Assay Kit Exclusive
Protein Carbonyl Assay Kit
Protein Carbonyl Fluorometric Assay Kit
Superoxide Dismutase Assay Kit
Thioredoxin Reductase Assay Kit
Xanthine Oxidase Assay Kit
Show all 25 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?