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Glutathione Peroxidase Assay Kit

Cayman Chemical Item Number 703102

GPx Assay Kit

Glutathione Peroxidase Assay Kit

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Glutathione Peroxidase Assay Kit Analysis Tools

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Description

Glutathione peroxidase (GPx) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPx, a monomer, all of the GPx enzymes are tetramers of four identical subunits.1,2 Each subunit contains a selenocysteine in the active site which participates directly in the two-electron reduction of the peroxide substrate.1,2 The enzyme uses glutathione as the ultimate electron donor to regenerate the reduced form of the selenocysteine.1,2 The Cayman Chemical Glutathione Peroxidase Assay Kit measures GPx activity indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of an organic hydroperoxide by GPx, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the A340 is directly proportional to the GPx activity in the sample.3 The Cayman GPx Assay Kit can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

1 Ceballos-Picot, I., Trivier, J., Nicole, A., et al. Age-correlated modifications of copper-zinc superoxide dismutase and glutathione-related enzyme activities in human erythrocytes. Clin Chem 38 66-70 (1992).

2 Forstrom, J.W., Zakowski, J.J., and Tappel, A.L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry 17 2639-2644 (1978).

3 Paglia, D.E., and Valentine, W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70 158-169 (1967).

Synonyms
  • GPx Assay Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Paglia, D.E., and Valentine, W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70 158-169 (1967).

Forstrom, J.W., Zakowski, J.J., and Tappel, A.L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry 17 2639-2644 (1978).

Ceballos-Picot, I., Trivier, J., Nicole, A., et al. Age-correlated modifications of copper-zinc superoxide dismutase and glutathione-related enzyme activities in human erythrocytes. Clin Chem 38 66-70 (1992).

703102-96WELL
96-Well Cover Sheet
96-Well Solid Plate (Colorimetric Assay)
GPx Assay Buffer (10X) 1 ea
GPx Sample Buffer (10X) 1 ea
Glutathione Peroxidase (Control) Assay Reagent 1 ea
GPx Co-Substrate Mixture 3 × 1 ea
GPx Cumene Hydroperoxide 1 ea
703102-480well
96-Well Cover Sheet
96-Well Solid Plate (Colorimetric Assay)
GPx Assay Buffer (10X) 2 × 1 ea
GPx Sample Buffer (10X) 1 ea
Glutathione Peroxidase (Control) Assay Reagent 1 ea
GPx Co-Substrate Mixture 5 × 5 ea
GPx Cumene Hydroperoxide 5 ea
Size Price Quantity Subtotal
96 wells $285.00 $0.00
480 wells $490.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glutathione Peroxidase Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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