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703102 Glutathione Peroxidase Assay Kit
GPx Assay Kit
Glutathione Peroxidase Assay Kit Workbook Links
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Glutathione peroxidase (GPx) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPx, a monomer, all of the GPx enzymes are tetramers of four identical subunits.1,2 Each subunit contains a selenocysteine in the active site which participates directly in the two-electron reduction of the peroxide substrate.1,2 The enzyme uses glutathione as the ultimate electron donor to regenerate the reduced form of the selenocysteine.1,2 The Cayman Chemical Glutathione Peroxidase Assay Kit measures GPx activity indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of an organic hydroperoxide by GPx, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the A340 is directly proportional to the GPx activity in the sample.3 The Cayman GPx Assay Kit can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
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1
Ceballos-Picot, I., Trivier, J., Nicole, A., et al. Age-correlated modifications of copper-zinc superoxide dismutase and glutathione-related enzyme activities in human erythrocytes. Clin Chem 38 66-70 (1992).
2
Forstrom, J.W., Zakowski, J.J., Tappel, A.L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry 17 2639-2644 (1978).
3
Paglia, D.E., Valentine, W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70 158-169 (1967).
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Purchase 703102 Glutathione Peroxidase Assay Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
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Warning This product is not for human or veterinary use.
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Pricing updated 2010-03-14.
Prices are subject to change without notice.
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