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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Glutathione Reductase Assay Kit

Cayman Chemical Item Number 703202

GR Assay Kit

Glutathione Reductase Assay Kit

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Description

Glutathione reductase (GR, EC 1.6.4.2) is a flavoprotein that catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to glutathione (GSH).1 This enzyme is essential for the GSH redox cycle which maintains adequate levels of reduced cellular GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. The Cayman Chemical Glutathione Reductase Assay Kit measures GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. Since GR is present at rate limiting concentrations, the rate of decrease in the A340 is directly proportional to the GR activity in the sample. The Cayman GR Assay Kit is provided in a convenient 96 well plate format and can be used to measure GR activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

1 Carlberg, I., and Mannervik, B. Glutathione reductase. Methods Enzymol 113 484-490 (1985).

Synonyms
  • GR Assay Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Carlberg, I., and Mannervik, B. Glutathione reductase. Methods Enzymol 113 484-490 (1985).

703202-96WELL
96-Well Solid Plate (Colorimetric Assay)
GR Assay Buffer (10X) 1 ea
GR Sample Buffer (10X) 1 ea
GR Glutathione Reductase (control) 1 ea
GR GSSG 1 ea
GR NADPH 3 × 1 ea
Size Price Quantity Subtotal
96 wells $225.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glutathione Reductase Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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