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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Filipin III

Cayman Chemical Item Number 70440

(CAS 480-49-9)

Filipin III (CAS 480-49-9)

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Description

Cholesterol is both an important structural component of cell membranes and an early intermediate in hormone and bile acid biosynthesis. The localization and measurement of cholesterol in cells is therefore of great interest. Filipin is the collective name given to four isomeric polyene macrolides isolated from cultures of S. filipinensis; Filipin III is the predominant isomer and the one used in most studies. Filipin binds to cholesterol in membranes, forming ultrastructural aggregates and complexes which can be visualized by freeze-fracture electron microscopy.1,2 The binding of cholesterol also decreases the intrinsic fluorescence of Filipin, and this property has also been used to detect cholesterol in membrane fractions.3

1 Castanho, M.A.R.B., Coutinho, A., and Prieto, M.J.E. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem 267 204-209 (1992).

2 Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes. Cell Biol Int Rep 8(7) 519-535 (1984).

3 Severs, N.J., and Robenek, H. Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochim Biophys Acta 737 373-408 (1983).

Formal Name 4S,​6S,​8S,​10R,​12R,​14R,​16S,​27S-​octahydroxy-​3R-​(1R-​hydroxy-​hexyl)17,​28R-​dimethyl-​oxacyclooctacosa-​17E,​119E,​21E,​21E,​23E,​25E-​pentaen-​2-​one
CAS Number 480-49-9
Molecular Formula C35H58O11
Formula Weight 654.8
Formulation A crystalline solid
Purity ≥85%
λmax 323, 339, 357 nm
Stability 1 year
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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CCCCC[C@@H]​(O)​[C@@H]​1[C@@H]​(O)​C[C@@H]​(O)​C[C@@H]​(O)​C[C@@H]​(O)​C[C@@H]​(O)​C[C@@H]​(O)​CC(O)​C(=CC=CC=CC=CC=CC(O)​[C@@H]​(C)​OC1=O)​C

Background Reading

Castanho, M.A.R.B., Coutinho, A., and Prieto, M.J.E. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem 267 204-209 (1992).

Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes. Cell Biol Int Rep 8(7) 519-535 (1984).

Severs, N.J., and Robenek, H. Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochim Biophys Acta 737 373-408 (1983).

Drabikowski, W., Lagwinska, E., and Sarzala, M.G. Filipin as a fluorescent probe for the location of cholesterol in the membranes of fragmented sarcoplasmic reticulum. Biochim Biophys Acta 291 61-70 (1973).

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Filipin III is available in the following screening library:

Size Price Quantity Subtotal
500 µg $56.00 $0.00
1 mg $106.00 $0.00
5 mg $448.00 $0.00
10 mg $784.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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