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TMPD (hydrochloride)

Cayman Chemical Item Number 70455

Wurster’s Reagent; N,N,N',N'-Tetramethyl-p-Phenylenediamine (CAS 637-01-4)

TMPD (hydrochloride) (CAS 637-01-4)

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Description

TMPD is an easily oxidizable compound that serves as a reducing co-substrate for heme peroxidases.1 TMPD undergoes one-electron oxidation by the heme peroxidase higher oxidation states (compounds I and II) producing a highly colored product that absorbs at 611 nm.2 Thus, the stoichiometry of oxidation is 2 moles of TMPD oxidized per mole of hydroperoxide reduced by the peroxidase. The extinction coefficient of the oxidized TMPD at 611 nm is 12,200.2 TMPD is also used for the detection of peroxidases on polyacrylamide gels.3

1 Van der Ouderaa, F.J., Buytenhek, M., Nugteren, D.H., et al. Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands. Biochim Biophys Acta 487 315-331 (1977).

2 Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

3 Butler, M.J., and Lachance, M.A. The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels. Anal Biochem 162 443-445 (1987).

Synonyms
  • Wurster's Reagent
  • N,N,N',N'-Tetramethyl-p-Phenylenediamine
Formal Name N,​N,​N',​N'-​tetramethyl-​1,​4-​benzenediamine,​ dihydrochloride
CAS Number 637-01-4
Molecular Formula C10H16N2 · 2HCl
Formula Weight 237.2
Formulation A crystalline solid
Purity >95%
λmax 263 nm
Stability 2 years
Storage Room temperature
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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CN(C)​c1ccc(cc1)​N(C)​C

Background Reading

Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

Van der Ouderaa, F.J., Buytenhek, M., Nugteren, D.H., et al. Purification and characterisation of prostaglandin endoperoxide synthetase from sheep vesicular glands. Biochim Biophys Acta 487 315-331 (1977).

Butler, M.J., and Lachance, M.A. The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels. Anal Biochem 162 443-445 (1987).
Size Price Quantity Subtotal
5 g $27.00 $0.00
10 g $51.00 $0.00
25 g $122.00 $0.00
50 g $216.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

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Pricing updated 2012-05-26. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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