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Lipid Hydroperoxide (LPO) Assay Kit (96 well)Cayman Chemical Item Number 705003 
Lipid Hydroperoxide (LPO) Assay Kit (96 well)
Description
Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.1,2,3 Lipid peroxidation results in the formation of highly reactive and unstable hydroperoxides of both saturated and unsaturated lipids. Our Lipid Hydroperoxide Assay Kit measures the hydroperoxides directly utilizing the redox reactions with ferrous ions.4 An easy to use quantitative extraction method was developed to extract lipid hydroperoxides into chloroform, and the extract is directly used in the assay. This procedure eliminates any interference caused by hydrogen peroxide or endogenous ferric ions in the sample and provides a sensitive and reliable assay for lipid peroxidation. This kit is available in two formats: 100 dtn, which is designed to be read using a single-
1 Cross, C.E., Halliwell, B., Borish, E.T., et al. Oxygen radicals and human disease. Ann Intern Med 107 526-545 (1987). 2 Halliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 25 57-74 (1996). 3 Porter, N.A., Mills, K.A., and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30 277-290 (1995). 4 Roomi, M.W., and Hopkins, C.Y. Some reactions of sterculic and malvalic acids. A new source of malvalic acid. Can J Biochem 48 759-762 (1970).
Background ReadingHalliwell, B. Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. Free Radic Res 25 57-74 (1996). Porter, N.A., Mills, K.A., and Caldwell, S.E. Mechanisms of free radical oxidation of unsaturated lipids. Lipids 30 277-290 (1995). Mihaljevic, B., Katusin-Razem, B., and Razem, D. The reevaluation of the ferric thiocyanate assay for lipid hydroperoxides with special considerations of the mechanism aspects of the response. Free Radic Biol Med 21 53-63 (1996). |
This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase. Pricing updated 2012-02-12. Prices are subject to change without notice. To ask for assistance with one of our products please contact a Technical Support Scientist. Warning This product is not for human or veterinary use. You may be eligible to receive a free sample Lipid Hydroperoxide (LPO) Assay Kit (96 well) under the Cayman Challenge program. Let Cayman analyze your samples for you. See EIA Service for details and availability. Related Products
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Batch-specific InformationLogin to access batch-specific information FAQsAre serum samples suitable for Lipid Hydroperoxide (LPO) Assay Kit? Yes, serum samples may be suitable for the LPO Assay Kit. In the kit booklet there is a procedure in the Sample Preparation for plasma which is suitable for serum also. Show answerIs there a protocol for serum sample preparation for the Lipid Hydroperoxide (LPO) Assay Kit? In the Lipid Hydroperoxide (LPO) Assay Kit booklet there is a procedure for sample preparation for plasma which is suitable for serum also. Show answer |
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins. |
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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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