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Catalase Assay KitCayman Chemical Item Number 707002 CAT 
Catalase Assay Kit
Catalase Assay Kit Analysis ToolsCayman offers a variety of analysis tools to assist you.
Description
Catalase (EC 1.11.1.6; 2H2O2 oxidoreductase) is an ubiquitous antioxidant enzyme that is present in most aerobic cells. Catalase (CAT) is involved in the detoxification of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), which is a toxic product of both normal aerobic metabolism and pathogenic ROS production. This enzyme catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While the aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. In humans, the highest levels of catalase are found in liver, kidney, and erythrocytes, where it is believed to account for the majority of hydrogen peroxide decomposition. The Cayman Chemical Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured spectrophotometrically with 4-
1 Johansson, L.H., and Borg, L.A.H. A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem 174 331-336 (1988). 2 Wheeler, C.R., Salzman, J.A., Elsayed, N.M., et al. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal Biochem 184 193-199 (1990).
Background ReadingWheeler, C.R., Salzman, J.A., Elsayed, N.M., et al. Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal Biochem 184 193-199 (1990). Johansson, L.H., and Borg, L.A.H. A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem 174 331-336 (1988). |
This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase. Pricing updated 2012-02-12. Prices are subject to change without notice. To ask for assistance with one of our products please contact a Technical Support Scientist. Warning This product is not for human or veterinary use. You may be eligible to receive a free sample Catalase Assay Kit under the Cayman Challenge program. Let Cayman analyze your samples for you. See EIA Service for details and availability. Related ProductsShow all 25 Hide all but first 3Downloads
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins. |
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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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