Antioxidant Assay Kit

Cayman Chemical Item Number 709001

Antioxidant Assay Kit

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Antioxidant Assay Kit Analysis Tools

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Description

The antioxidant system of living organisms includes enzymes such as superoxide dismutase, catalase, and glutathione peroxidase; macromolecules such as albumin, ceruloplasmin, and ferritin; and an array of small molecules, including ascorbic acid, α-tocopherol, β-carotene, reduced glutathione, uric acid, and bilirubin. The sum of endogenous and food-derived antioxidants represents the total antioxidant activity of the extracellular fluid. Cooperation of all the different antioxidants provides greater protection against attack by reactive oxygen or nitrogen radicals, than any single compound alone. Thus, the overall antioxidant capacity may give more relevant biological information compared to that obtained by the measurement of individual components, as it considers the cumulative effect of all antioxidants present in plasma and body fluids. The Cayman Chemical Antioxidant Assay Kit measures the total antioxidant capacity of plasma, serum, urine, saliva, or cell lysates. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS^® (2,2'-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS^® · + by metmyoglobin.^1,2,3,4 The capacity of the antioxidants in the sample to prevent ABTS oxidation is compared with that of Trolox, a water-soluble tocopherol analogue, and is quantified as molar Trolox equivalents. Each kit contains assay buffer (10X), chromogen, metmyoglobin, trolox, hydrogen peroxide, a 96 well plate, plate cover, and complete instructions.

1 Miller, N., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84 407-412 (1993).

2 Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS+ radical cation assay . Free Radic Res 26 195-199 (1997).

3 Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity. Biochem Soc Trans 21 95S (1993).

4 Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids. Methods Enzymol 234(24) 279-293 (1994).

Stability

1 year

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Miller, N.J., and Rice-Evans, C. Factors influencing the antioxidant activity determined by the ABTS+ radical cation assay . Free Radic Res 26 195-199 (1997).

Miller, N., Rice-Evans, C., Davies, M.J., et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84 407-412 (1993).

Rice-Evans, C., and Miller, N. Total antioxidant status in plasma and body fluids. Methods Enzymol 234(24) 279-293 (1994).

Miller, N.J., Rice-Evans, C., and Davies, M.J. A new method for measuring antioxidant activity. Biochem Soc Trans 21 95S (1993).

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709001-96WELL

Antioxidant Assay Buffer (10X)

1 ea

Antioxidant Assay Chromogen

3 × 1 ea

Antioxidant Assay Metmyoglobin

2 × 1 ea

Antioxidant Assay Trolox

3 × 1 ea