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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

COX Activity Assay Kit

Cayman Chemical Item Number 760151

COX Activity Assay Kit

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COX Activity Assay Kit Analysis Tools

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Description

The COX Activity Assay utilizes the peroxidase component of cyclooxygenases. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) at 590 nm.1 The assay can be used to measure COX activity (COX-1 and COX-2) in cell lysates, tissue homogenates, and purified enzyme preparations. The assay includes COX-1 and COX-2 specific inhibitors in order to distinguish between the two isozymes.

1 Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Kulmacz, R.J., and Lands, W.E.M. Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase. Prostaglandins 25 531-540 (1983).

760151-96WELL
96-Well Solid Plate (Colorimetric Assay)
COX Standard 1 ea
Arachidonic Acid Assay Reagent 1 ea
Assay Buffer (10X) 1 ea
Potassium Hydroxide Assay Reagent 1 ea
Heme Assay Reagent 1 ea
Colorimetric Substrate 1 ea
DuP-697 Assay Reagent 1 ea
SC-560 Assay Reagent 1 ea
Size Price Quantity Subtotal
96 wells $209.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample COX Activity Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Batch-specific Information

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FAQs

In the COX Activity Assay Kit, what is the principle of the reaction of TMPD by peroxidase?

In the Background section of the COX Activity Assay Kit booklet, it states that the COX component converts arachidonic acid to Prostaglandin G2 (PGG2) and the peroxidase component reduces the PGG2 to the corresponding alcohol, PGH2. In this kit the TMPD serves as a reducing agent, TMPD gets oxidized, so electrons flow from the TMPD to PGG2 and the appearance of the oxidized. TMPD is monitored at 590 nm.

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