Lipoxygenase Inhibitor Screening Assay Kit

Cayman Chemical Item Number 760700

LO Inhibitor Screening Assay Kit

Lipoxygenase Inhibitor Screening Assay Kit

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Lipoxygenase Inhibitor Screening Assay Kit Analysis Tools

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Description

Lipoxygenases are members of a class of non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system to give an unsaturated fatty acid hydroperoxide. In mammals, lipoxygenases carry out the first step in the arachidonic acid cascade.^1,2 5- and 15-LOs lead to the biologically active lipoxins, whereas 5-LO leads to 5,6-epoxy-leukotrienes which are involved in a variety of inflammatory responses, including neutrophil chemotaxis, vascular permeability, and smooth muscle contraction.³ In contrast, Nassar, et al., suggested that animal 15-LO products act as anti-inflammatory agents.⁴ This implies that the 5- and 15-LO pathways may play a role in regulating inflammation. To elucidate the role of each lipoxygenase, it is particularly important to develop inhibitors that are enzyme specific. This assay kit provides an accurate and convenient method for screening lipoxygenase inhibitors. This assay measures the hydroperoxides generated from the incubation of a lipoxygenase (5-, 12-, or 15-LO) with either arachidonic or linoleic acid.

1 Gaffney, B.J. Lipoxygenases: Structural principles and spectroscopy. Annu Rev Biophys Biomol Struct 25 431-459 (1996).

2 Yamamoto, S. Mammalian lipoxygenases: Molecular structures and functions. Biochim Biophys Acta 1128 117-131 (1992).

3 Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

4 Nassar, G.M., Morrow, J.D., Roberts, L.J., et al. Induction of 15-lipoxygenase by interleukin-13 in human blood monocytes. J Biol Chem 269 27631-27634 (1994).

Synonyms

LO Inhibitor Screening Assay Kit

Stability

1 year

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Nassar, G.M., Morrow, J.D., Roberts, L.J., et al. Induction of 15-lipoxygenase by interleukin-13 in human blood monocytes. J Biol Chem 269 27631-27634 (1994).

Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

Yamamoto, S. Mammalian lipoxygenases: Molecular structures and functions. Biochim Biophys Acta 1128 117-131 (1992).

Gaffney, B.J. Lipoxygenases: Structural principles and spectroscopy. Annu Rev Biophys Biomol Struct 25 431-459 (1996).

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760700-96WELL

96-Well Solid Plate (Colorimetric Assay)