PAF Acetylhydrolase Assay Kit

Cayman Chemical Item Number 760901

PAF-AH; Lp-PLA₂

PAF Acetylhydrolase Assay Kit

See analysis tools…

PAF Acetylhydrolase Assay Kit Analysis Tools

Cayman offers a variety of analysis tools to assist you.

See the PAF Acetylhydrolase Assay Kit workbook.
See a list of all analysis workbooks

Description

Platelet-activating factor (PAF) is a biologically active phospholipid synthesized by a variety of cells upon stimulation. PAF is converted to the biologically inactive lyso-PAF by the enzyme PAF acetylhydrolase (PAF-AH). Plasma PAF-AH is highly selective for phospholipids with very short acyl groups at the sn-2 position and is associated with lipoproteins.¹ The Cayman Chemical PAF acetylhydrolase assay kit provides an accurate and convenient method for measurement of PAF-AH activity. The assay uses 2-thio PAF which serves as a substrate for PAF-AH.² Upon hydrolysis of the acetyl thioester bond at the sn-2 position by PAF-AH, free thiols are detected using 5,5 · -dithiobis(2-nitrobenzoic acid) (DTNB; Ellman’s reagent). The dynamic range of the assay is only limited by the accuracy of the absorbance measurement. Most plate readers are linear to an absorbance of 1.2. The detection range of the assay is from 0.02 to 0.2 µmol/min/ml of PAF-AH activity which is equivalent to an absorbance increase of 0.01 to 0.1 per minute. Each kit contains assay buffer, DTNB, 2-thio PAF (substrate), human PAF-AH standard, a 96 well plate, and complete instructions.

1 Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. J Biol Chem 262 4223-4230 (1987).

2 Aarsman, A.J., Neys, F.W., and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. Eur J Biochem 200 187-193 (1991).

Synonyms

PAF-AH
Lp-PLA2

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Stafforini, D.M., Prescott, S.M., and McIntyre, T.M. Human plasma platelet-activating factor acetylhydrolase. J Biol Chem 262 4223-4230 (1987).

Aarsman, A.J., Neys, F.W., and van den Bosch, H. Catabolism of platelet-activating factor and its acyl analog. Differentiation of the activities of lysophospholipase and platelet-activating-factor acetylhydrolase. Eur J Biochem 200 187-193 (1991).

760901-96WELL

96-Well Solid Plate (Colorimetric Assay)