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Nitrate/Nitrite Colorimetric Assay Kit

Cayman Chemical Item Number 780001

Nitric Oxide Metabolite Detection Kit

Nitrate/Nitrite Colorimetric Assay Kit

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Nitrate/Nitrite Colorimetric Assay Kit Analysis Tools

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Description

The Cayman Chemical Nitrate/Nitrite Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step is the addition of the Griess Reagents which convert nitrite into a deep purple azo compound. Photometric measurement of the absorbance due to this azo chromophore accurately determines nitrite concentration. This kit can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media, and tissue homogenates. However, it cannot be used to analyze nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH has been added. For these assays a second method (LDH method) is utilized and is available from Cayman Chemical in a 96 well plate format (Catalog No. 760871).
Synonyms
  • Nitric Oxide Metabolite Detection Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
780001-192WELL
96-Well Solid Plate (Colorimetric Assay)
Nitrate Reductase Enzyme Preparation 2 × 1 ea
Nitrate Reductase Cofactor Preparation 2 × 1 ea
NO Assay Nitrate Standard 1 ea
Nitrite Standard 1 ea
Griess Reagent R1 2 × 1 ea
Griess Reagent R2 2 × 1 ea
Nitrate/Nitrite Assay Buffer 1 ea
Size Global Purchasing
2 x 96 wells See a distributor in your region for pricing

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Nitrate/Nitrite Colorimetric Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Is this kit right for my sample type? Nitrate/Nitrite Kit Recommendation Chart

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FAQs

What are the main differences between the Nitrate/Nitrite Colorimetric Assay Kit and Nitrate/Nitrite Colorimetric Assay Kit (LDH method)?

The Nitrate/Nitrite Colorimetric Assay , product number 780001, uses a NADPH cofactor recycling method which allows use of a NADPH concentration that is below the level of interference in the Griess reaction. The Nitrate/Nitrite Colorimetric Assay (LDH Method), product number 790871 uses LDH to eliminate all excess NADPH that is required for NOS activity assays. The LDH kit also removes excess NADPH used to catalyze conversion of Nitrate to Nitrite. Of the two nitrate/nitrite assays mentioned, we recommend the Nitrate/Nitrite Colorimetric Assay (or the corresponding Fluorometric assay if greater sensitivity is needed) for most applications since it is significantly easier to perform. The only reason to use the Nitrate/Nitrite Colorimetric Assay Kit (LDH method) is for measurement of Nitrate/nitrite from in vitro NOS activity assays.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

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