Nitrate/Nitrite Fluorometric Assay Kit

Cayman Chemical Item Number 780051

Nitric Oxide Metabolite Detection Kit

Nitrate/Nitrite Fluorometric Assay Kit

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Nitrate/Nitrite Fluorometric Assay Kit Analysis Tools

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See the Nitrate/Nitrite Fluorometric Assay Kit workbook.
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See more	Assay Kits (331) Small Molecule Detection (Non-EIA) (30) Nitric Oxide (101)

Description

Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t1/2 = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO₂-) and nitrate (NO₃-). Relative proportions of NO₂- and NO₃- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical’s Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO₂- and NO₃- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO₂/NO₃ is ~50 nM.

Synonyms	Nitric Oxide Metabolite Detection Kit
Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

780051-192WELL
96-Well Solid Plate (white)
Nitrate Reductase Enzyme Preparation	2 × 1 ea
Nitrate Reductase Cofactor Preparation	2 × 1 ea
NO Assay Nitrate Standard	1 ea
Nitrite Standard	1 ea
Nitrate/Nitrite Assay Buffer	1 ea
Sodium Hydroxide (2.8 M)	1 ea
DAN Reagent	1 ea

Size	Global Purchasing
2 x 96 wells	See a distributor in your region for pricing

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Nitrate/Nitrite Fluorometric Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Is this kit right for my sample type? Nitrate/Nitrite Kit Recommendation Chart

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Downloads

	Download Kit Booklet 1090 Kb PDF
	Download Material Safety Data Sheet (MSDS) 79 Kb PDF
	Printer-friendly version of this page small PDF (usually less than 100 Kb)
	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm
	Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Nitrate/Nitrite Fluorometric Assay Kit

Nitrate/Nitrite Fluorometric Assay Kit

Nitrate/Nitrite Fluorometric Assay Kit Analysis Tools

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