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DAN-1 EE (hydrochloride)

Cayman Chemical Item Number 85070

DAN-1 EE (hydrochloride)

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Description

DAN-1 EE is a fluorescent indicator for the bioimaging of nitric oxide (NO).1 DAN-1 EE is a cell permeable derivative of DAN, a molecule which has been used extensively in the quantitation of nitrate and nitrite using fluorescence spectroscopy.2,3 Upon entry into the cell, DAN-1 EE is transformed into the less cell permeable DAN-1 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. Intracellular formation of NO can be monitored using excitation and emission wavelengths of 360-380 nm and 420-450 nm, respectively.1

1 Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for the bioimaging of nitric oxide. Biol Pharm Bull 20 1229-1232 (1997).

2 Miles, A.M., Chen, Y., Owens, M.W., et al. Fluorometric determination of nitric oxide. Methods 7 40-47 (1995).

3 Misko, T.P., Schilling, R.J., Salvemini, D., et al. A fluorometric assay for the measurement of nitrite in biological samples. Anal Biochem 214 11-16 (1993).

Formal Name 4-​[[(3-​amino-​2-​naphthalenyl)amino]methyl]-​benzoic acid,​ ethyl ester,​ monohydrochloride
Molecular Formula C20H20N2O2 · HCl
Formula Weight 356.9
Formulation A solution in ethanol
Purity ≥95%
λmax 251 nm
Stability 1 year
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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NC1=CC2=CC=CC=C2C=C1NCC3=CC=C(CC(OC)​=O)​C=C3.Cl

Background Reading

Misko, T.P., Schilling, R.J., Salvemini, D., et al. A fluorometric assay for the measurement of nitrite in biological samples. Anal Biochem 214 11-16 (1993).

Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for the bioimaging of nitric oxide. Biol Pharm Bull 20 1229-1232 (1997).

Miles, A.M., Chen, Y., Owens, M.W., et al. Fluorometric determination of nitric oxide. Methods 7 40-47 (1995).
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