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Dihydrorhodamine 123

Cayman Chemical Item Number 85100

DHR (CAS 109244-58-8)

Dihydrorhodamine 123 (CAS 109244-58-8)

See more	Fluorescence (90) Reactive Probes (27)

Description

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation.¹ It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR.¹ Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M⁻¹cm⁻¹).^2,3,1 DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.^4,5,6

1 Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1 145-157 (1997).

2 Briviba, K., Roussyn, I., Sharov, V.S., et al. Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen. Biochem J 319 13-15 (1996).

3 Sies, H., Sharov, V.S., Klotz, L., et al. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase. J Biol Chem 272 27812-27817 (1997).

4 Handa, O., Stephen, J., and Cepinskas, G. Role of endothelial nitric oxide synthase-derived nitric oxide in activation and dysfunction of cerebrovascular endothelial cells during early onsets of sepsis. Am J Physiol Heart Circ Physiol 295 H1712-9 (2008).

5 Lacy, P., Latif, D.A., Steward, M., et al. Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutophils from atopic subjects. J Immunol 170 2670-9 (2003).

6 Li, J., Baud, O., Volpe, J.J., et al. Peroxynitrite generated by inducible nitric oxide synthase and NADPH oxidase mediates microglial toxicity to oligodendrocytes. Proc Natl Acad Sci USA 102(28) 9936-9941 (2005).

Synonyms	DHR
Formal Name	2-(3,6-diamino-9H-xanthen-9-yl)-benzoic acid, methyl ester
CAS Number	109244-58-8
Molecular Formula	C₂₁H₁₈N₂O₃
Formula Weight	346.4
Formulation	A crystalline solid
Purity	≥98%
λ_max	223, 289 nm
Stability	2 years
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere
SMILES	Copy SMILES to clipboard COC(=O)c1ccccc1C1c2ccc(N)cc2Oc2cc(N)ccc12

Background Reading

Briviba, K., Roussyn, I., Sharov, V.S., et al. Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen. Biochem J 319 13-15 (1996).

Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1 145-157 (1997).

Sies, H., Sharov, V.S., Klotz, L., et al. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase. J Biol Chem 272 27812-27817 (1997).

Size	Price	Quantity	Subtotal
1 mg	$26.00		$0.00
5 mg	$117.00		$0.00
10 mg	$208.00		$0.00
25 mg	$455.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-02-12. Prices are subject to change without notice.

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Related Products

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Peroxynitrite

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