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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

2,7-Dichlorodihydrofluorescein diacetate

Cayman Chemical Item Number 85155

DCDHF diacetate; DCF (CAS 4091-99-0)

2,7-Dichlorodihydrofluorescein diacetate (CAS 4091-99-0)

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Description

DCDHF (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation.1,2 DCDHF is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCDHF. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).1,2

1 Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1 145-157 (1997).

2 Kooy, N.W., Royall, J.A., and Ischiropoulos, H. Oxidation of 2',7'-dichlorofluorescin by peroxynitrite. Free Radic Res 27 245-254 (1997).

Synonyms
  • DCDHF diacetate
  • DCF
Formal Name 2-​(2,​7-​dichloro-​3,​6-​diacetyloxy-​9H-​xanthen-​9-​yl)-​benzoic acid
CAS Number 4091-99-0
Molecular Formula C24H16Cl2O7
Formula Weight 487.3
Formulation A crystalline solid
Purity ≥95%
λmax 258 nm
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
SMILES
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CC(=O)​Oc1cc2Oc3cc(OC(=O)​C)​c(Cl)​cc3C(c2cc1Cl)​c1ccccc1C(=O)​O

Background Reading

Kooy, N.W., Royall, J.A., and Ischiropoulos, H. Oxidation of 2',7'-dichlorofluorescin by peroxynitrite. Free Radic Res 27 245-254 (1997).

Possel, H., Noack, H., Augustin, W., et al. 2,7-dihydrodichlorofluorescein diacetate as a fluorescent marker for peroxynitrite formation. FEBS Lett 416 175-178 (1997).

Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: Implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1 145-157 (1997).
Size Price Quantity Subtotal
50 mg $26.00 $0.00
100 mg $49.00 $0.00
250 mg $117.00 $0.00
500 mg $208.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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