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DAF-2

Cayman Chemical Item Number 85160

4,5-Diaminofluorescein (CAS 205391-01-1)

DAF-2 (CAS 205391-01-1)

See more	Fluorescence (90) Reactive Probes (27)

Description

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).^1,2 It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.² At neutral pH the detection limit for NO is 2-5 nM.

1 Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem Pharm Bull 46 373-375 (1998).

2 Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Lett 427 263-266 (1998).

Synonyms	4,5-Diaminofluorescein
Formal Name	2-(3,6-dihydroxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid
CAS Number	205391-01-1
Molecular Formula	C₂₀H₁₄N₂O₅
Formula Weight	362.3
Formulation	A solution in DMSO
Purity	≥98%
Stability	1 year
Storage	-20°C
Shipping	Room temperature in continental US; may vary elsewhere
SMILES	Copy SMILES to clipboard Oc1ccc2c(Oc3c(ccc(O)c3N)C2c2ccccc2C(=O)O)c1N

Background Reading

Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem Pharm Bull 46 373-375 (1998).

Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Lett 427 263-266 (1998).

Size	Global Purchasing
100 µg	See a distributor in your region for pricing
250 µg	See a distributor in your region for pricing
500 µg	See a distributor in your region for pricing
1 mg	See a distributor in your region for pricing

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Warning This product is not for human or veterinary use.

Related Products

DAF-2 diacetate

DAN-1 EE (hydrochloride)

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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