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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

DAF-2 diacetate

Cayman Chemical Item Number 85165

4,5-Diaminofluorescein diacetate (CAS 205391-02-2)

DAF-2 diacetate (CAS 205391-02-2)

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Description

DAF-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO).1,2 It is a cell-permeable derivative of DAF-2. Upon entry into the cell, DAF-2 diacetate is transformed into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively.2 At neutral pH the detection limit for NO is 2-5 nM. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amount of NO, such as macrophages.1,2

1 Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem Pharm Bull 46 373-375 (1998).

2 Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Lett 427 263-266 (1998).

Synonyms
  • 4,5-Diaminofluorescein diacetate
Formal Name 2-​(3,​6-​diacetyloxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid
CAS Number 205391-02-2
Molecular Formula C24H18N2O7
Formula Weight 446.4
Formulation A solution in DMSO
Purity ≥95%
Stability 1 year
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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CC(=O)​Oc1ccc2c(Oc3c(N)​c(ccc3C2c2ccccc2C(=O)​O)​OC(=O)​C)​c1N

Background Reading

Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem Pharm Bull 46 373-375 (1998).

Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Lett 427 263-266 (1998).
Size Price Quantity Subtotal
100 µg $42.00 $0.00
250 µg $100.00 $0.00
500 µg $189.00 $0.00
1 mg $336.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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