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Nitrotyrosine BSACayman Chemical Item Number 89542
Peroxynitrite-
Description
This product can be used as a positive control for immunoblotting experiments using Cayman Chemical’s Nitrotyrosine Polyclonal Antibody (Catalog No. 189540) and Nitrotyrosine Monoclonal Antibody (Catalog No. 189542). Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide (6.7 x 109 M−1sec−1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.1,2 Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including atherosclerotic lesions of human coronary arteries, postischemic heart, and placenta during preeclampsia.3,4,5 Increased nitration of proteins in motor neurons has been identified in patients with amyotrophic lateral sclerosis and may be due to mutations in superoxide dismutase.2,6,7,8
1 Beckman, J.S., Chen, J., Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods Enzymol 233 229-240 (1994). 2 Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. Am J Physiol 271 C1424-C1437 (1996). 3 Beckman, J.S., Ye, Y.Z., Anderson, P.G., et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe-Seyler 375 81-88 (1994).
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Wang, P., and Zweier, J.L. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite- 5 Myatt, L., Rosenfield, R.B., Eis, A.L.W., et al. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 28 488-493 (1996).
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Abe, K., Pan, L., Watanabe, M., et al. Upregulation of protein- 7 Chou, S.M., Wang, H.S., and Komai, K. Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: An immunohistochemical study. J Chem Neuroanat 10 249-258 (1996).
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Ferrante, R.J., Shinobu, L.A., Schulz, J.B., et al. Increased 3-
Background ReadingBeckman, J.S., Ye, Y.Z., Anderson, P.G., et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe-Seyler 375 81-88 (1994). Chou, S.M., Wang, H.S., and Komai, K. Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: An immunohistochemical study. J Chem Neuroanat 10 249-258 (1996). Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. Am J Physiol 271 C1424-C1437 (1996). Ferrante, R.J., Shinobu, L.A., Schulz, J.B., et al. Increased 3- Abe, K., Pan, L., Watanabe, M., et al. Upregulation of protein- Wang, P., and Zweier, J.L. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite- Myatt, L., Rosenfield, R.B., Eis, A.L.W., et al. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 28 488-493 (1996). Beckman, J.S., Chen, J., Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods Enzymol 233 229-240 (1994). Show all 8 Hide all but first 3 |
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