GSHases Analysis Tools

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This is the Cayman Chemical Company Glutathione Enzyme (GSHases) Assay Tools page. This area is dedicated to serving the analysis needs of our Assay Kit customers. It contains information, Excel workbooks, and other tools useful for anyone using the Cayman Chemical Glutathione Peroxidase, Glutathione Reductase and Glutathione S-Transferase Assays.

If you have questions on the use of our tools, or feel something is missing, please contact Technical Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing GSHases assays: GSHasesTriple and GSHasesDouble. The first is formatted for assays performed in triplicate, and the second is formatted for assays performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in each workbook.

• GSHasesTriple ~ 600 Kb XLS formatted for assays performed in triplicate
• GSHasesDouble ~ 600 Kb XLS formatted for assays performed in duplicate

Trouble downloading? Right-click the link and select "Save Target As..."

These instructions are repeated on the first sheet of each workbook.

GSHasesTriple Worksheet

GSHasesDouble worksheet

Definition of Key Terms

Workbook Sheets

Analysis

Graphs

These are the kinetic curves for each sample. The reaction time is plotted versus the absorbance of the sample at 340 nm.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

1. Select, from the pull down menu, which enzyme assay you are running
2. Input your sample names into the appropriate cells
3. Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)
4. Paste/type your data into the TIME #:## sections (starting in cells B12, B21, B30, B39, B48 & B57) of the Analysis worksheet. Select the "Values" option in the "Paste Special" dialog box to maintain formatting

All activity values, and relevant statistics, are automatically calculated. The RESULTS table is then filled in with the data. All averages and statistics are calculated based on all replicates for each sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the presence of outliers in 96-well based assays. We find that individual replicates are rarely flagged as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the individual spreadsheets.

Copyright and Disclaimer

Copyright © 2012 Cayman Chemical Company, Inc. All rights reserved.

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

1. Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.
2. Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and should not be interpreted as representing official policies, either expressed or implied, of Cayman Chemical Company, Inc.