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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Lipoxygenase Inhibitor Screening (LIS) Analysis Tools

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If you have questions on the use of our tools, or feel something is missing, please contact Technical Services.

The Workbooks

Your downloading these files implies acceptance of the license agreement listed below and present in each workbook.

  • LISTriple ~ 600 Kb XLS formatted for assays performed in triplicate
  • LISDouble ~ 600 Kb XLS formatted for assays performed in duplicate

Trouble downloading? Right-click the link and select "Save Target As..."

These instructions are repeated on the first sheet of each workbook.

LISTriple worksheet screen shot
LISTriple Worksheet
LISDouble worksheet screen shot
LISDouble worksheet

Definition of Key Terms

Term Definition
BLANK Serves as a negative control
Positive Ctrl Positive control provided in the kit
100% Init. Activity Initial activity of the 15-lipoxygenase standard provided in the kit
Smpl# (1:#) Samples and their associated dilution factors

Workbook Sheets

Analysis
Field Explanation
LAYOUT Contains sample names and associated dilution factors
RAW DATA Absorbance value (between 490-500 nm) of each well after two 5 minute incubations
ACTIVITY For each absorbance value, the activity of the well is calculated using the method on page 15 of the kit booklet
RESULTS This table is compiled from the preceding sections. It includes the sample name, assay dilution and percent inhibition of the compound. The inhibition is calculated using the method on page 16 in the kit booklet
FILENAME The filename given to the Excel workbook
VERSION The version of the workbook

Instructions

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the individual spreadsheets.

Copyright and Disclaimer

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