Cayman Chemical | Analysis | Nitrate/Nitrite (LDH)

Nitrate/Nitrite (LDH) Analysis Tools

This is the Cayman Chemical Company Nitrate/Nitrite Colorimetric (LDH Method) Assay Tools page. This area is dedicated to serving the analysis needs of our Nitrate/Nitrite Colorimetric (LDH Method) Kit customers. It contains information, Excel workbooks, and other tools useful for anyone using the Cayman Chemical Nitrate/Nitrite Assay Kit.

If you have questions on the use of our tools, or feel something is missing, please contact Technical Services.

The Workbooks

Currently, Cayman Chemical uses two Microsoft Excel workbooks for analyzing Nitrate/Nitrite assays: NO2NO3Triple and NO2NO3Double. The first is formatted for assays performed in triplicate, and the second is formatted for assays performed in duplicate.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in each workbook.

NO2NO3LDHTriple ~ 600 Kb XLS formatted for assays performed in triplicate
NO2NO3LDHDouble ~ 600 Kb XLS formatted for assays performed in duplicate

Trouble downloading? Right-click the link and select "Save Target As..."

These instructions are repeated on the first sheet of each workbook.

NO2NO3LDHTriple Worksheet

NO2NO3LDHDouble worksheet

Definition of Key Terms

Term	Definition
StdX (Standards)	Standards A through F prepared according to the kit protocol
Smpl# (1:#)	Samples and their associated dilution factors
Ctrl# (1:#)	Positive controls and their dilutions (Cayman Chemical uses 1:5 dilutions of StdF & StdE)

Workbook Sheets

Total & Nitrite

LAYOUT	Contains sample names and associated dilution factors
RAW DATA	Data entered into the worksheet in the format defined by the LAYOUT section
SUBTRACTED	All absorbance values with the AvgBlnk subtracted
CONC	The concentration for each cell is calculated based on the fluorescence intensity value, constants from the linear regression curve (see StdCurve section), sample volume, and dilution factor from the LAYOUT section
STANDARDS	The Avg absorbance values are calculated for each concentration
Samples	This table is compiled from the preceding sections. It includes Conc: average of the replicate concentrations from the CONC section Dilution: the dilution factor, for that sample, from the LAYOUT section StdDev: the standard deviation of the replicate concentration values %CV: the coefficient of variance of the replicate concentration values SEM: the standard error of the mean for the replicate concentration values
STATS	y-int and slope are obtained from the linear regression fit of the calibration data
FILENAME	The filename given to the Excel workbook
VERSION	The version of the workbook

StdCurve

This is the calibration curve of the concentration standards (Total and Nitrite) plotted versus their absorbance values. The data are fit to a linear regression line with the general equation: y = mx + b

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you have loaded your plate according to one of the plate layouts shown on the Workbooks sheet, and have the data in a form that can be imported (or pasted) directly into a spreadsheet. The following instructions apply to both the Total and Nitrite worksheets. To analyze your data:

Input your sample names into the appropriate cells
Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)
Paste/type your data into the RAW DATA section (starting in cell B12) of the Total and Nitrite worksheet. Select the "Values" option in the "Paste Special..." dialog box to maintain formatting

All concentration values, and relevant statistics, are automatically calculated. The Samples table is then filled in with the data. All averages and statistics are calculated based on all replicates for each sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the presence of outliers in 96-well based assays. We find that individual replicates are rarely flagged as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.
Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and should not be interpreted as representing official policies, either expressed or implied, of Cayman Chemical Company, Inc.