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SOD Activity Analysis Tools

This is the Cayman Chemical Company SOD Activity Tools page. It contains information, Excel workbooks, and other tools useful for anyone performing SOD Activity assays.

If you have questions on the use of our tools, or feel something is missing, please contact Technical Services.

The Workbooks

Currently, Cayman Chemical uses two Excel workbooks to perform SOD Activity analysis. Both are available for your download and use.

Download

Your downloading these files implies acceptance of the license agreement listed below and present in each workbook.

SODTriple ~ 600 Kb XLS formatted for assays performed in triplicate
SODDouble ~ 600 Kb XLS formatted for assays performed in duplicate

Trouble downloading? Right-click the link and select "Save Target As..."

These instructions are repeated on the first sheet of each workbook.

SODTriple Worksheet

SODDouble worksheet

Definition of Key Terms

Term	Definition
Std# (Standards)	Standards A through H prepared as outlined in the kit booklet.
Ctrl# (1:#)	Positive controls and their dilutions (Cayman Chemical uses 1:2 dilutions of StdF & StdH)
Smpl# (1:#)	Samples and their associated dilution factors

Workbook Sheets

Analysis

Field	Explanation
LAYOUT	Contains sample names and associated dilution factors
RAW DATA	Data entered into the worksheet in the format defined by the LAYOUT section. Data greater than the highest standard (ULOQ) or less than the lowest standard (LLOQ) are highlighted.
LINRZD RATE	For each cell, Blank is divided by the cell absorbance to give the linearized rate
ACTIVITY	The activity for each cell is calculated using the linearized rate, constants from the linear-regression curve, and the dilution factor from the LAYOUT section.
STANDARDS	The average linearized rates (Avg LR) are calculated for each activity value
Samples	This table is compiled from the preceding sections. It includes U/mL: average of the replicate activities from the ACTIVITY section. Dilution: the dilution factor, for that sample, from the LAYOUT section StdDev: the standard deviation of the replicate concentration values SD: the standard deviation of the replicate activity values. %CV: the coefficient of variance of the replicate activity values.
STATS	slope, y-int, and R2 are obtained from the linear-regression fit of the standard curve data.

StdCurve

This is the calibration curve of the standard activities plotted versus their linearized rates. The data are fit to a linear-regression line with the general equation: y = m x + b.

Instructions

The worksheets were designed to be as "hands-off" as possible. The following instructions assume you have loaded your plate according to one of the plate layouts shown above, and have the data in a form that can be imported (or pasted) directly into a spreadsheet. To analyze your data:

Input your sample names into the appropriate cells
Input the sample dilutions into the appropriate cells in the format shown (e.g., 1:5)
Paste/type your data into the RAW DATA section (starting in cell B12) of the EndPoint worksheet. Select the “Values” option in the “Paste Special…” dialog box to maintain formatting

All activity values, and relevant statistics, are automatically calculated. The Samples table is then filled in with the data. All averages and statistics are calculated based on all replicates for each sample. It is the responsibility of the individual researcher to decide if any data can be eliminated. Caution should be exercised, however, when doing this! Cayman Chemical has examined statistical methods to test for the presence of outliers in 96-well based assays. We find that individual replicates are rarely flagged as outliers, and cannot be eliminated from the experiment.

Note The sole purpose of the data contained in this workbook is to demonstrate the functions of the individual spreadsheets.

Copyright and Disclaimer

Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:

Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer.
Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution.

THIS SOFTWARE IS PROVIDED BY CAYMAN CHEMICAL COMPANY, INC. "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL CAYMAN CHEMICAL BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

The views and conclusions contained in the software and documentation are those of the authors and should not be interpreted as representing official policies, either expressed or implied, of Cayman Chemical Company, Inc.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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