QWhat sample types can be used with the S-Nitrosylation Protein Detection Kit?

AThe protocol in the S-Nitrosylated Protein Detection Kit (cat. 10006518) refers to use of a cell pellet which can be obtained from cultured cells, or blood cells. Alternatively, the assay can be used with purified protein. If using a purified protein, begin by resuspending the protein sample and incubating at 2mg/mL in Buffer A containing Blocking Reagent; this will keep the blocking reagent in excess. At this point, free thiols on the protein are blocked and you can continue with the acetone precipitations in step 6. For gel analysis, load only 1-5 µg of protein per lane to avoid overloading the protein.