High Throughput Screening Center

Conventional immunoassay techniques requiring long washing, precipitation, scintillation counting, or other complex steps have largely been supplanted in target drug evaluation by homogeneous, single step assays. This fast, preliminary analysis or High Throughput Screening (HTS) has the potential to identify the most promising chemical entities among tens of thousands of candidate compounds.

However, if the HTS method sacrifices sensitivity or accuracy in the pursuit of speed, much of its promise is wasted.

Cayman HTS Products

Cayman's HTS assays are built to meet the standards required by the HTS community including:

1. No significant loss of sensitivity relative to conventional immunoassay
2. Minimal levels of matrix interference
3. Quick, single-step assay protocols of 60 minutes or less

The result is that we offer high-quality assays that are often a fraction of the cost per well of a conventional assay—for example, the PGE₂ FPIA - Green is only $0.41 per well in a 384-well format.

Fluorescence Polarization Assays

Cayman currently offers Fluorescence Polarization Immunoassays (FPIAs) for the measurement of Prostaglandin E₂ and Prostaglandin D₂ which are based on either fluorescein (“Green”) or rhodamine (“Red”) fluorophores. In addition, a novel FP-based assay for hematopoietic-type Prostaglandin D Synthase is now available.

Please contact Cayman Chemical's sales or technical staff to see how these assays fit into your current or future drug screening campaign.

FPIAs

• Introduction to FPIA

Class	Rhodamine “Red”	Fluorescein “Green”	Non-FPIA counterpart
Prostaglandin E2	Prostaglandin E2 FPIA Kit - Red 384 wells @ $305.00 all sizes… Description and pricing Validation Poster 560 Kb PDF	Prostaglandin E2 FPIA Kit - Green 384 wells @ $161.00 all sizes… Description and pricing Validation	Prostaglandin E2 EIA Kit - Monoclonal 96 solid wells @ $270.00 all sizes… Description and pricing
Prostaglandin D2	$product.getDisplayNameMarkup() 96 solid wells @ $270.00 all sizes… Description and pricing	Prostaglandin D2 FPIA Kit - Green 384 wells @ $305.00 all sizes… Description and pricing Poster 932 Kb PDF	$product.getDisplayNameMarkup() 384 wells @ $305.00 all sizes… Description and pricing

Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

Cayman’s H-PGDS FP-Based Inhibitor Screening Assay Kit - Green provides a rapid, accurate assay for screening H-PGDS inhibitors. The novel technology in this assay eliminates the need for using the highly unstable substrate PGH₂. Rather, an H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme.

• Full information for Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green...

Screening Libraries

Cayman Chemical offers a unique set of compound libraries, rich in biologically active molecules, useful for screening and hit-seeking for diverse therapeutic targets.