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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday.
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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.
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STEP UP to Better Cell-Based Assays |
Introducing ready-to-use STEP (Surface Transfection and Expression Protocol) assays. STEP technology lets you simply plate cells and have functional assays ready to go in a day or two!
| Item № | Product | Plain Text Name | Keywords |
|---|---|---|---|
| 600340 | EP2 Receptor (rat) STEP Reporter Assay Kit (Luminescence) Exclusive | EP2 Receptor (rat) STEP Reporter Assay Kit (Luminescence) | prostaglandins E2 PGE2 prostanoids G protein-coupled receptors GPCRs EP1 EP3 EP4 signal transduction tissues regulation cells mediates cAMP cyclic messenger pathways mucosal protection gastrointestinal secretions motility immunity inflammation mice mouse murine tumors cancers cancer-associated immunodeficiency dendritic-cell differentiation in vitro neuroprotective NMDA toxicity oxygen glucose deprivation agonists antagonists therapeutics Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening |
| 600410 | EP4 Receptor (rat) STEP Plate Assay Kit (cAMP method) | EP4 Receptor (rat) STEP Plate Assay Kit (cAMP method) | cell-based assays cells CBAs kits measures measurements prostaglandins E2 PGE2 prostanoids G protein-coupled receptors proteins GPCRs EP1 EP2 EP3 signals transductions mechanisms tissues localizations regulation expression intestines lungs kidneys thymus uterus brains cyclics AMP adenosines monophosphates pathways phosphatidylinositols 3-kinases G1 smooth muscles relaxation gastric secretions intestinal epithelial transportation adrenal aldosterone uterine immunity inflammation human colon cancers colorectal carcinogenesis in vivo neuroprotective excitotoxic injury injuries agonists antagonists therapeutics diseases surface transfections and expression protocols transient technology technologies University of Michigan UM U of M Originus, Inc. USPT United States patents 6897067 6902933 7056741 DNA deoxyribonucleic recombinant proteins cells receptor-mediated endocytosis solution-phase efficiency multiple plasmids constructs intracellular |
| 600350 | EP4 Receptor (rat) STEP Reporter Assay Kit (Luminescence) | EP4 Receptor (rat) STEP Reporter Assay Kit (Luminescence) | prostaglandins E2 PGE2 prostanoids G protein-coupled receptors GPCRs EP1 EP2 EP3 signal transduction tissues regulation cells intestines lungs kidneys thymus brains uterus mediates cAMP cyclic messenger pathways PtdIns phosphatidylinositols 3-kinases Gi mediates relaxation smooth muscles gastric acid secretions intestinal epithelial transportation adrenal aldosterone uterine immunity inflammation mice mouse murine colon tumors cancers cancer-associated carcinogenesis in vivo neuroprotective NMDA neuroprotective excitotoxic brain injury agonists antagonists therapeutics Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening |
| 600180 |
Melanocortin- |
Melanocortin-3 Receptor STEP Reporter Assay Kit (Luminescence) | MC-3R melanocortins petides hormones proopiomelanocortins pituitary CNS central nervous systems skin placenta tissues MCR receptors G protein-coupled GPCRs cAMP Adenosine 3',5' cyclic mononucleotides messenger signal transduction pathways melanocortin-3 hypothalmus homeostasis fat lean mass hyperphagia MC4R melanocortin-4 adiposity MC3R-knockout mice mouse murine fatty acids oxidations blood pressure hypertension obesity glucose metabolisms insulin insensitivity agonists ligands Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening a-MSH alpha-MSH α-MSH |
| 600190 |
Melanocortin- |
Melanocortin-4 Receptor STEP Reporter Assay Kit (Luminescence) | MC-4R melanocortins petides hormones proopiomelanocortins pituitary CNS central nervous systems skin placenta tissues MCR receptors G protein-coupled GPCRs cAMP Adenosine 3',5' cyclic mononucleotides messenger signal transduction pathways melanocortin-3 hypothalmus homeostasis fat lean mass hyperphagia MC4R melanocortin-4 adiposity MC3R-knockout mice mouse murine fatty acids oxidations blood pressure hypertension obesity glucose metabolisms insulin insensitivity agonists ligands sexual function antagonists fever suppression inflammatory mediators in vivo Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated endocytosis solution-phase co-transfection plasmids secretory alkalines phosphatases substrates SEAP proteins lipids signal transduction luminescence-based cell-based assays kits CBAs high-throughput screening a-MSH alpha-MSH α-MSH |
| 600240 | Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence) Exclusive | Orexin 1 Receptor STEP Reporter Assay Kit (Luminescence) | hypercretins 1 orexins 2 A orexin-2 systems regulates regulator sleep wake cycles feeding cardiovascular autonomic hypothalamic neuropeptides Orexins A Orexins B gut hormones secretions hypothalamus midbrains brainstems G protein-coupled receptors GPCRs orexin-1 OX1R orexin-2 OX2R orexin-A orexin-B sleep disorders narcolepsy humans animals rodents energy homeostasis pain stress antagonists drugs addictions withdrawals therapeutics obesity Surface transfection expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated luminescent secretory alkaline phosphatases SEAP signal transduction cascades culture medium luminescence-based high throughput screening screens agonists |
| 600250 | Orexin 2 Receptor STEP Reporter Assay Kit (Luminescence) Exclusive | Orexin 2 Receptor STEP Reporter Assay Kit (Luminescence) | hypercretins 1 orexin 2 systems regulates regulator sleep wake cycles feeding cardiovascular autonomic hypothalamic neuropeptides Orexins A Orexins B gut hormones secretions hypothalamus midbrains brainstems G protein-coupled receptors GPCRs orexin-1 OX1R orexin-2 OX2R orexin-A orexin-B sleep disorders narcolepsy humans animals rodents energy homeostasis pain stress antagonists drugs addictions withdrawals therapeutics obesity Surface transfection and expression protocols solid phase transient transfection technology originus patents USPT US6897067 US6902933 US7056741 US 6897067 6902933 7056741 DNA deoxyribonucleases adherent cells receptor-mediated luminescent secretory alkaline phosphatases SEAP signal transduction cascades culture medium luminescence-based high throughput screening screens agonists |
Consistently high transfection and co-transfection efficiency making reliable and robust assays possible.
- Convenient ready to assay or store up to one year
- Low Cost use routine cell lines, no need to maintain multiple stable cell lines
- Reliable and Robust consistently high transfection and co-transfection efficiency
- Highly Reproducible low variability well-to-well, plate-to-plate, batch-to-batch
- Multiple Readouts calcium flux, ELISA, reporter assays, high content, signaling pathways
- Allow Side-By-Side Comparison eliminate variations among individual stable cell lines
- All Assay Reagents Included in the Kit
STEP: Surface Transfection and Expression Protocol
Stable cell lines are difficult to generate particularly when multiple expression constructs are involved. In addition, it is not possible to modulate the stoichiometry of transgene expression and the property of the stable cell line may change over time. Transient (solution-based) transfection has its advantages, but it is not compatible with many cell lines, and it has a limited co-transfection efficiency. STEP (Surface Transfection and Expression Protocol) technology has overcome many of the disadvantages of conventional transient and stable transfection approaches. It involves the formation of a transfection complex applied directly on the growing surface of culture vessels. This enhances both the transfection and co-transfection efficiencies in many cell lines and even primary cultures. The STEP complex is evenly coated on multi-well plates. The homogenous coating of STEP complex provides superior transfection efficiency consistently from well to well, which is essential in an assay for high throughput screening.
Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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