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Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday.
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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.
Protocols
Plant
| Sample | Bead Type | Speed | Cycles | Cycle Length(s) | Tested Applications |
|---|---|---|---|---|---|
| Arabidopsis | Ceramic (2.8 mm) | 6500 | 2 | 20 | RNA/Cells extraction |
| Grape fruit | Metal (2.8 mm) | 6500 | 2 | 30 | |
| Mosses | Metal (2.8 mm) | 6500 | 1 | 30 | DNA extraction |
| Nuts | Ceramic (1.4 mm) | 6500 | 2 | 20 | |
| Peanuts | Ceramic (2.8 mm) | 6500 | 1 | 40 | |
| Pepper | Metal (2.8 mm) | 6500 | 1 | 40 | |
| Seaweed | Glass (0.5 mm) | 6000 | 2 | 50 | |
| Spinach leaf root | Ceramic or Metal (2.8 mm) | 6500 | 3 | 45 | |
| Tomato peel | Metal (2.8 mm) | 6500 | 3 | 45 | |
| Tomato pulp | Ceramic (2.8 mm) | 6500 | 2 | 30 | |
| Wheat embryo | Ceramic (2.8 mm) | 6500 | 2 | 30 | |
| Wheat leaf | Ceramic (2.8 mm) | 6500 | 2 | 45 | |
| Wheat root | Ceramic (2.8 mm) | 6500 | 3 | 45 |
Micro-organisms
| Sample | Bead Type | Speed | Cycles | Cycle Length(s) | Tested Applications |
|---|---|---|---|---|---|
| Bacillus subtilis | Glass (0.5 mm) | 6500 | 2 | 23 | Vitamin isolation/HPLC |
| Bacillus subtilis | Glass (0.5 mm) | 6500 | 3 | 45 | RNA Isolation to make cDNA |
| Bacteria solution | Glass (0.5 mm)s | 5500 | 3 | 30 | Protein extraction |
| E. coli | Glass (0.1 mm) | 6000 | 2 (with 60s break) | 20 | Protein extraction |
| Spores | Glass (0.5 mm) | 6000 | 2 | 30 |
Tissue (animal)
| Sample | Bead Type | Speed | Cycles | Cycle Length(s) | Tested Applications |
|---|---|---|---|---|---|
| Animal lymph nodes | Ceramic (2.8 mm) | 6000 | 2 | 50 | DNA extraction/PCR |
| Animal placentas | Ceramic (2.8 mm) | 6000 | 1 | 30 | DNA extraction/PCR |
| Animal spleen | Ceramic (2.8 mm) | 6000 | 1 | 30 | DNA extraction/PCR |
| Artery | Ceramic (2.8 mm | 6000 | 2 | 50 | Relaxometry |
| Bovine brain | Ceramic (1.4 mm) | 6500 | 2 | 30 | Prion protein extraction |
| Bovine lymphoid tissues | Ceramic (1.4 mm) | 6500 | 3 | 30 | Prion protein extraction |
| Fish gonad | Ceramic (1.4 mm) | 6000 | 3 | 10 | ELISA |
| Fish liver | Ceramic (1.4 mm) | 6000 | 3 | 10 | ELISA |
| Frog skin | Ceramic (2.8 mm) | 6200 | 2-3 | 50 | DNA extraction/PCR |
| Murine aorta | Ceramic (2.8 mm) | 5000 | 2 | 30 | Protein extraction for ELISA or DNA extraction/PCR |
| Murine brain | Ceramic (1.4 mm) | 5000 | 1 | 10 | Isolation of DNA |
| Murine brain | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of RNA |
| Murine brain | Ceramic (2.8 mm) | 5500 | 1 | 20 | HPLC MS/MS |
| Murine ear | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of DNA |
| Murine ear | Ceramic (2.8 mm) | 5000 | 2 | 20 | Isolation of RNA |
| Murine heart | Ceramic (1.4 mm) | 5000 | 1 | 15 | Isolation of DNA |
| Murine heart | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of RNA |
| Murine heart | Ceramic or Metal (2.8 mm) | 6500 | 2 | 30 | HPLC MS/MS |
| Murine kidney | Ceramic (1.4 mm) | 5000 | 1 | 15 | Isolation of DNA |
| Murine kidney | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of RNA |
| Murine liver | Ceramic (1.4 mm) | 5000 | 1 | 15 | Isolation of DNA |
| Murine liver | Ceramic (1.4 mm) | 5000 | 1 | 10 | Isolation of RNA |
| Murine liver | Ceramic (2.8 mm) | 6000 | 1 | 30 | HPLC MS/MS |
| Murine liver | Ceramic or Metal (2.8 mm) | 5000 | 2 | 50 | Protein extraction for ELISA |
| Murine lung | Ceramic (1.4 mm) | 5000 | 1 | 15 | Isolation of DNA |
| Murine lung | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of RNA |
| Murine lung | Ceramic (2.8 mm) | 6000 | 2 | 30 | HPLC MS/MS |
| Murine lung | Ceramic or Metal (2.8 mm) | 5000 | 2 | 50 | Protein extraction for ELISA |
| Murine muscle | Ceramic (1.4 mm) | 5000 | 1 | 10 | Isolation of DNA |
| Murine muscle | Ceramic (1.4 mm) | 5000 | 1 | 20 | Isolation of RNA |
| Murine muscle | Ceramic or Metal (2.8 mm) | 6000 | 2 | 30 | HPLC MS/MS |
| Murine salivary gland | Ceramic (2.8 mm) | 5000 | 2 | 50 | Protein extraction for ELISA |
| Murine salivary gland | Ceramic (2.8 mm) | 6000 | 2 | 50 | DNA extraction/PCR |
| Murine spleen | Ceramic (1.4 mm) | 5000 | 1 | 15 | Isolation of DNA |
| Murine tail | Ceramic (1.4 mm) | 6500 | 3 | 20 | Isolation of RNA |
| Rat heart | Ceramic (1.4 mm) | 6500 | 2 | 8 | Enzymatic dosage |
| Rat heart | Ceramic (1.4 mm) | 6500 | 2 | 15 | Drug concentration |
| Rat tail | Ceramic (2.8 mm) | 6500 | 3 | 20 | RNA extraction |
| Sheep | Ceramic or Metal (2.8 mm) | 6000 | 2 | 30 | DNA extraction/PCR |
| Virus from tissues (heart, spleen, pancreas) | Ceramic (2.8 mm) | 6500 | 3 | 5 | RNA extraction |
Tissues (human)
| Sample | Bead Type | Speed | Cycles | Cycle Length(s) | Tested Applications |
|---|---|---|---|---|---|
| Blood traces | Ceramic or Metal (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PCR |
| Blood traces on special paper (FTA) | Metal (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PCR |
| Breast tumor | Ceramic (1.4 mm) | 5500 | 1 | 20 | RNA extraction |
| Hair | Metal (2.8 mm) | 6500 | 2 | 50 | HPLC MS/MS |
| Human bones | Ceramic (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PCR |
| Human colon | Ceramic (1.4 mm) | 5000 | 2 | 50 | Western blot |
| Human kidney | Ceramic (1.4 mm) | 5000 | 2 | 50 | Western blot |
| Human liver | Ceramic or Metal (2.8 mm) | 6500 | 2 | 50 | Forensic/Toxicology |
| Human lung | Ceramic or Metal (2.8 mm) | 6500 | 2 | 50 | Forensic/Toxicology |
| Human mamma-CA | Ceramic (1.4 mm) | 5000 | 2 | 50 | Protein arrays |
| Human muscle | Ceramic or Metal (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PCR |
| Human pancreas | Ceramic (1.4 mm) | 5000 | 2 | 50 | Western blot |
| Human skin biopsies | Ceramic (1.4 mm) | 6300 | 6 | 23 | RNA extraction |
| Human Teeth | Metal (2.8 mm) | 6500 | 3 | 50 | DNA extraction |
| Mouth mucus | Ceramic or Metal (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PCR |
| Virus from tissues (heart, spleen, pancreas) | Ceramic (2.8 mm) | 6500 | 3 | 5 | RNA extraction |
Other
| Sample | Bead Type | Speed | Cycles | Cycle Length(s) | Tested Applications |
|---|---|---|---|---|---|
| Blood traces on clothes | Ceramic (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PC |
| Chewing gum | Ceramic (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PC |
| Cigarette filter | Ceramic (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PC |
| Small cigar filter | Ceramic (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PC |
| Trace sample on cotton bud | Metal (2.8 mm) | 6500 | 2 | 50 | DNA extraction/PC |
| Paracetamol | Metal (2.8 mm) | 5000 | 3 | 10 | Microniaztion |
Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA
Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640
Copyright 2012 Cayman Chemical Company
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