Our kits use acetylcholinesterase (AChE) as the enzymatic label. AChE has the fastest turnover rate of any enzymatic label. AChE assays are developed with Ellman's Reagent, which contains acetylthiocholine as the substrate. The final product of the enzymatic reaction, 5-thio-2-nitrobenzoic acid, is bright yellow and can be read at 405-420 nm.
AChE offers several advantages over other enzymes conventionally used in ELISAs: