Leptin (human) EIA Kit
Item № 500010
96 wells $457.00 $0.00

Pricing updated 2016-05-02. Prices are subject to change without notice.

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  • Measure leptin in human serum, plasma, and culture supernatant samples
  • Assay 41 samples in duplicate
  • Incubation: 2 x 1 hour
  • Leptin (human) ELISA Kit

Leptin, the product of the ob (obese) gene, is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism, and body weight regulation. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice suggesting that endogeneous leptin can normalize their body weight. In contrast, human obese subjects may have high level of leptin, indicating a mechanism of leptin resistance. This Enzyme Immunometric Assay (EIA) is based on a double-antibody sandwich technique. The wells of the plate supplied with the kit are coated with a polyclonal antibody specific of human leptin. This antibody will bind any Leptin introduced in the wells (sample or standard).

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Download Kit Booklet

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Technical Information
  • Leptin (human) ELISA Kit
  • (100%) human Leptin
  • (<0.1%) bovine Leptin
  • (<0.1%) equine Leptin
  • (<0.1%) goat Leptin
  • (<0.1%) murine Leptin
  • (<0.1%) ovine Leptin
  • (<0.1%) porcine Leptin
  • (<0.1%) rabbit Leptin
  • (<0.1%) rat Leptin
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WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 9 months from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
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Additional Information

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Analysis Tools

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