Cortisol ELISA Kit
Item № 500360
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00

Pricing updated 2016-04-29. Prices are subject to change without notice.

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  • Measure cortisol in urine, plasma, and other sample matrices
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure cortisol levels down to 35 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • Cortisol EIA Kit

Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol ELISA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The assay has a range from 6.6-4,000 pg/ml and a sensitivity (80% B/B0) of approximately 35 pg/ml.

NOTE: Due to an approximate 15% cross-reactivity with dexamethasone, this kit may not be suitable for use with samples that contain high concentrations of dexamethasone, such as those collected during a dexamethasone suppression test.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS)

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Technical Information
  • Cortisol EIA Kit
Assay Range 6.6-4,000 pg/ml
Sensitivity 80% B/B0: 35 pg/ml
Mid-point 50% B/B0: 150-210 pg/ml
  • (100%) Cortisol
  • (4.0%) Prednisolone
  • (1.6%) Cortexolone
  • (0.23%) 11-Deoxycorticosterone
  • (0.23%) 17-Hydroxyprogesterone
  • (0.15%) Cortisol Glucuronide
  • (0.14%) Corticosterone
  • (0.13%) Cortisone
  • (<0.01%) Androstenedione
  • (<0.01%) Enterolactone
  • (<0.01%) Estrone
  • (<0.01%) 17-Hydroxypregnenolone
  • (<0.01%) Pregnenolone
  • (<0.01%) Testosterone
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WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

QC Sheets
Certificates of Analysis
References & Background Reading
Background Reading

Masuzaki, H., Paterson, J., Shinyama, H., et al. A transgenic model of visceral obesity and the metabolic syndrome. Science 294 2166-2170 (2001).}

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).}

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).}

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).}

Varghese, F.P., and Brown, E.S. The hypothalamic-pituitary-adrenal axis in major depressive disorder: A brief primer for primary care physicians. Prim Care Companion J Clin Psychiatry 3 151-155 (2001).}

Krieger, D.T. Rhythms of ACTH and corticosteroid secretion in health and disease, and their experimental modification. J Steroid Biochem 6 785-791 (1975).}

Rask, E., Olsson, T., Söderberg, S., et al. Tissue-specific dysregulation of cortisol metabolism in human obesity. J Clin Endocrinol Metab 86(3) 1418-1421 (2001).}

Stulnig, T.M., and Waldhäusl, W. 11.beta.-Hydroxysteroid dehydrogenase type 1 in obesity and type 2 diabetes. Diabetologia 47 1-11 (2004).}

Aardal, E., and Holm, A. Cortisol in saliva-reference ranges and relation to cortisol in serum. Eur J Clin Chem Clin Biochem 33 927-932 (1995).}

Quinkler, M., and Stewart, P.M. Hypertension and the cortisol-cortisone shuttle. J Clin Endocrinol Metab 88(6) 2384-2392 (2003).}

Christeff, N., Michon, C., Goertz, G., et al. Abnormal free fatty acids and cortisol concentrations in the serum of AIDS patients. Eur J Cancer Clin Oncol 24(7) 1179-1183 (1988).}

Brown, E.S., Varghese, F.P., and McEwen, B.S. Association of depression with medical illness: Does cortisol play a role? Biol Psychiatry 55 1-9 (2004).}

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Cortisol ELISA Kit

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View the pre-configured analysis tool for Cortisol ELISA Kit on

Contact Us
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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research.

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You may be eligible to receive a free sample of Cortisol ELISA Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA