Estradiol ELISA Kit
Item № 582251
SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 solid wells $185.00 $0.00
96 strip wells $185.00 $0.00
480 solid wells $694.00 $0.00
480 strip wells $694.00 $0.00

Pricing updated 2016-05-24. Prices are subject to change without notice.

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Description
Features
  • Measure estradiol (17β-estradiol) in cell culture medium, plasma, and serum
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure estradiol levels down to 15 pg/ml
  • Incubation: 1 hour | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
Synonyms
  • Estradiol EIA Kit
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol

Estradiol (17β-estradiol) is the major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female genotype in embryogenesis and at puberty. In addition, estradiol is an important luteolytic agent in humans.1 Estradiol is synthesized from testosterone primarily in the ovarian granulosa cells and placenta, but small amounts can be produced in the adrenal gland.2,3 The conversion of testosterone to estradiol is accomplished by the aromatase system, which consists of 3 enzyme activities localized to the endoplasmic reticulum of the cells in these tissues.1,4 Plasma estradiol levels increase gradually between days 1-7 of the menstrual cycle followed by a sharp increase to a peak value of about 300 pg/ml on day 12 just prior to ovulation.1,5 Estradiol levels in most rodent samples are extremely low, rising to detectable levels only during the pro-estrus period and in pregnant females.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS) Find Batch-Specific Documents

Related Products
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Technical Information
Synonyms
  • Estradiol EIA Kit
  • E2
  • β-Estradiol
  • 17β-Estradiol
  • 17β-Oestradiol
Assay Range 6.6-4,000 pg/ml
Sensitivity 80% B/B0: ~15 pg/ml
Mid-point 50% B/B0: ~40-100 pg/ml
Specificity
  • (100%) Estradiol
  • (14.5%) Estradiol-3-sulfate
  • (14%) Estradiol-3-glucuronide
  • (12%) Estrone
  • (10%) Estradiol-17-glucuronide
  • (0.30%) Estriol
  • (0.06%) 5α-dihydro Testosterone
  • (0.05%) Ethynyl Estradiol
  • (0.02%) 5-Androstan-17β-ol-3-one
  • (0.02%) Androstenediol
  • (<0.01%) Aldosterone
  • (<0.01%) Cortisol
  • (<0.01%) 17α-Estradiol
  • (<0.01%) Estradiol-3-benzoate
  • (<0.01%) Estradiol-17-glucoronidate
  • (<0.01%) Estradiol-17-sulfate
  • (<0.01%) Hydrocortisone
  • (<0.01%) Maturation Inducing Steroid (salmonid)
  • (<0.01%) Progesterone
  • (<0.01%) 17α-hydroxy Progesterone
  • (<0.01%) Testosterone
  • (0.008%) DHEA
  • (0.0024%) DHEA Sulfate
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WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, and GC-MS data.

Inserts
Please enter your batch number(s) to access inserts
QC Sheets
Please enter your batch number(s) to access QC sheets
Certificates of Analysis
Please enter your batch number(s) to access Certificates of Analysis
GC-MS Data
Please enter your batch number(s) to access GC-MS data
References & Background Reading
Product Description References

1. Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).

2. Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).

3. Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).

4. Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).

5. Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).

Background Reading

Vance, D.E. Cholesterol and related derivatives. 2nd ed. 725-748 (1988).}

Muñoz, N.M., Kim, K.P., Han, S.K., et al. Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A2 (hVPLA2). Hybridoma 19 171-176 (2000).}

Erickson, G.F. The ovary: Basic principles and concepts. A. Physiology. 3rd ed. 973-1015 (1995).}

Naka, M., Mais, D.E., Morinelli, T.A., et al. 7-[(1R,2S,3S,5R)-6,6-Dimethyl-3-(4-iodobenzenesulfonylamino)bicyclo[3.1.1]hept-2-yl]-5(Z)-heptenoic acid: A novel high-affinity radiolabeled antagonist for platelet thromboxane A2/prostaglandin H2 receptors. J Pharmacol Exp Ther 262 632-637 (1992).}

Munro, C.J., Stabenfeldt, G.H., Cragun, J.R., et al. Relationship of serum estradiol and progesterone concentrations to the excretion profiles of their major urinary metabolites as measured by enzyme immunoassay and radioimmunoassay. Clin Chem 37 838-844 (1991).}

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).}

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).}

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).}

Miller, W.L., and Tyrrell, J.B. The adrenal cortex. 3rd edition 555-711 (1995).}

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for Estradiol ELISA Kit

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View the pre-configured analysis tool for Estradiol ELISA Kit on MyAssays.com

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  • Direct Phone: (734) 975-3888
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