DNA/RNA Oxidative Damage ELISA Kit
Item № 589320
96 solid wells $495.00 $0.00
96 strip wells $495.00 $0.00
480 solid wells $1,856.00 $0.00
480 strip wells $1,856.00 $0.00

Pricing updated 2016-05-04. Prices are subject to change without notice.

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  • Measure major oxidative damage markers, 8-hydroxy 2’-deoxyguanosine​​, 8-hydroxyguanosine, and 8-hydroxyguanine in urine, cell culture medium, cell lysates, tissue samples, saliva, and plasma/serum samples
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Assay Range: 10.3-3,000 pg/ml
  • Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm
  • DNA/RNA Oxidative Damage ELISA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG

Both DNA and RNA are damaged by oxidation during aging and in a variety of disease states including cancer. The repair processes that are initiated to correct this damage release multiple oxidized guanine species into the urine. Cayman Chemical has developed this immunoassay for the measurement of DNA/RNA oxidative damage that detects all three oxidized guanine species; 8-hydroxy-2'-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA, and 8-hydroxyguanine from either DNA or RNA. Some commercial vendors offer immunoassays that detect only 8-hydroxy-2'-deoxyguanosine, and not the other two molecules. The advantage of the Cayman kit is that it captures a more complete set of biologically relevant products of oxidative damage than do assays that are restricted to analysis of only 8-hydroxy-2'-deoxyguanosine. However, because Cayman’s kit recognizes more than 8-hydroxy-2'-deoxyguanosine, it is not valid to compare the results from the Cayman ELISA Kit to an LC/MS analysis of 8-hydroxy-2'-deoxyguanosine. The ELISA value will always be significantly higher than LC/MS because the ELISA also detects 8-hydroxyguanosine and 8-hydroxyguanine.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS)

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Technical Information
  • DNA/RNA Oxidative Damage ELISA Kit
  • 8-hydroxy-2'-Deoxyguanosine
  • 8-OH-DG
  • 8-OH-2dG
Assay Range 10.3-3,000 pg/ml
Sensitivity 80% B/B0: ~30 pg/ml
Mid-point 50% B/B0: ~90-140 pg/ml
  • (100%) 8-hydroxy-2’-deoxyguanosine
  • (38%) 8-hydroxyguanosine
  • (23%) 8-hydroxyguanine
  • (<0.01%) Guanosine
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WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Abbreviated Kit Protocol

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

QC Sheets
Certificates of Analysis
References & Background Reading
Background Reading

Baldridge, K.C., Zavala, J., Surratt, J., et al. Cellular RNA is chemically modified by exposure to air pollution mixtures. Inhal Toxicol 27 74-82 (2015).}

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).}

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).}

Gan, W., Nie, B., Shi, F., et al. Age-dependent increases in the oxidative damage of DNA, RNA, and their metabolites in normal and senescence-accelerated mice analyzed by LC-MS/MS: Urinary 8-oxoguanosine as a novel biomarker of aging. Free Radic Biol Med 52 1700-1707 (2012).}

Shi, F., Nie, B., Gan, W., et al. Oxidative damage of DNA, RNA and their metabolites in leukocytes, plasma and urine of Macaca mulatta: 8-oxoguanosine in urine is a useful marker for aging. Free Radic Res 46(9) 1093-1098 (2012).}

Roszkowski, K., and Olinski, R. Urinary 8-oxoguanine as a predictor of survival in patients undergoing radiotherapy. Cancer Epidemiol Biomarkers Prev 21 629-634 (2012).}

Weimann, A., Broedbaek, K., Henriksen, T., et al. Assays for urinary biomarkers of oxidatively damaged nucleic acids. Free Radic Res 46(4) 531-540 (2012).}

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).}

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Analysis Tools

View our analysis workbook for DNA/RNA Oxidative Damage ELISA Kit

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View the pre-configured analysis tool for DNA/RNA Oxidative Damage ELISA Kit on MyAssays.com


The the level of 8-OH-dG has been estimated in cultured cells after extracting total DNA. What is the best way to normalize the data for accurate comparison between treatments?

It is more meaningful to normalize measured values to the total number of cells, or the amount of purified DNA rather than as a concentration of 8-OH-dG only.

Has the 8-hydroxy-2-deoxy Guanosine EIA Kit been validated for muscle tissue or whole blood (leukocyte DNA) samples?

The 8-hydroxy-2-deoxy Guanosine EIA Kit has not been validated for those exact sample types, but has been validated for cell lysates and tissue homogenates in general. Theoretically, the kit should work just fine with any purified DNA sample.

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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

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Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA