Thiol Detection Assay Kit
Item № 700340
96 wells $178.00 $0.00
480 wells $668.00 $0.00

Pricing updated 2016-05-06. Prices are subject to change without notice.

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  • Measure free thiol content in plasma, serum, urine, cell lysates, and tissue homogenates
  • Assay 40 samples in duplicate
  • Assay Range: 15 nm-1 µM
  • Plate-based fluorometric measurement (ex 380-390 nm, em 510-520 nm)
  • Includes both a glutathione and cysteine standard

The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection Assay Kit provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS) Find Batch-Specific Documents

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Technical Information
Limit of Detection 15 nM

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, and GC-MS data.

Please enter your batch number(s) to access inserts
QC Sheets
Please enter your batch number(s) to access QC sheets
Certificates of Analysis
Please enter your batch number(s) to access Certificates of Analysis
GC-MS Data
Please enter your batch number(s) to access GC-MS data
References & Background Reading
Background Reading

Dickinson, D.A., and Forman, H.J. Cellular glutathione and thiols metabolism. Biochem Pharmacol 64 1019-1026 (2002).}

Wendel, A., and Cikryt, P. The level and half-life of glutathione in human plasma. FEBS Lett 120 209-211 (1980).}

Wlodek, L. Beneficial and harmful effects of thiols. Pol J Pharmacol 54 215-223 (2002).}

Lash, L.H., and Jones, D.P. Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch Biochem Biophys 240 583-592 (1985).}

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Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

Contact Us
  • To streamline the process attach the appropriate questionnaire to your inquiry.

  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research.

Cayman is know as a partner to scientists worldwide, responding to your needs with services in:


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You may be eligible to receive a free sample of Thiol Detection Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA