L-Lactate Assay Kit
Item № 700510
96 wells $325.00 $0.00

Pricing updated 2016-05-05. Prices are subject to change without notice.

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  • Measure L-lactate in biological samples such as serum, plasma, whole blood, urine, saliva, and cell culture
  • Assay 40 samples in duplicate
  • Assay Range: 25 μM-1 mM
  • Plate-based, fluorometric measurement (ex 530-540 nm, em 585-595 nm)

L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH). Monitoring lactate levels is, therefore, a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Cayman’s L-Lactate Assay provides a fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, lactate dehydrogenase catalyzes the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS)

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Technical Information

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 6 months from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

QC Sheets
Certificates of Analysis
References & Background Reading
Background Reading

Gladden, L.B. Lactate metabolism: A new paradigm for the third millenium. J Physiol 558(1) 5-30 (2004).}

Passarella, S., de Bari, L., Daniela, V., et al. Mitochondria and L-lactate metabolism. FEBS Lett 582 3569-3576 (2008).}

Bellomo, R. Bench-to-bedside review: lactate and the kidney. Crit Care 6(4) 322-326 (2002).}

McLellan, A.C., Phillips, S.A., and Thornally, P.J. Flourimetric assay of D-lactate. Anal Biochem 206(1) 12-16 (1992).}

Luft, F.C. Lactic acidosis update for critical care clinicians. J Am Soc Nephrol 12 S15-S19 (2001).}

Robergs, R.A., Ghiasvand, F., and Parker, D. Biochemistry of exercise-induced metabolic acidosis. Am J Physiol Regul Integr Comp Physiol 287(3) R502-R516 (2004).}

Du, W.B., Pan, X.P., and Li, L.J. Prognostic models for acute liver failure. Hepatobiliary Panceat Dis Int 9(2) 122-8 (2010).}

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1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA