Hemoglobin Colorimetric Assay Kit
Item № 700540
2 x 96 wells $215.00 $0.00

Pricing updated 2016-05-24. Prices are subject to change without notice.

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  • Measure total hemoglobin concentration in blood, tissue homogenates, and cell lysates
  • Assay 40 samples in duplicate
  • Assay Range: 0.016-0.4 g/dl
  • Plate-based, colorimetric measurement (560-590 nm)
  • Hb
  • Hgb

Hemoglobin (Hb or Hgb) is a globular protein found primarily in erythrocytes that carries oxygen from the lungs to tissue where it releases oxygen and then returns carbon dioxide (CO2) from the tissue to the lungs. Beyond binding and transporting oxygen, hemoglobin also binds CO2, carbon monoxide (CO), and nitric oxide (NO). Cayman’s Hemoglobin Colorimetric Assay provides a quick, reliable method for determining total hemoglobin concentration in a variety of biological samples, including blood, tissue homogenates, and cell lysates. Unlike the universally accepted reference method of hemoglobin determination, which uses potassium cyanide as a reagent and commonly under-estimates hemoglobin levels, Cayman’s Hemoglobin Assay utilizes an optimized detection reagent which is non-toxic, reporting accurate measurements of total hemoglobin concentrations with an absorbance between 560-590 nm.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Let us analyze your samples for you. See our contract research services.

Download Kit Booklet Download Safety Data Sheet (SDS) Find Batch-Specific Documents

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Technical Information
  • Hb
  • Hgb
Limit of Detection 3 µM (0.005 g/dl)

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere
Stability As supplied, 1 year from the QC date provided on the Certificate of Analysis, when stored properly
Product Downloads & Resources
Product Downloads

Download Kit Booklet

Download Safety Data Sheet (SDS)

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, and GC-MS data.

Please enter your batch number(s) to access inserts
QC Sheets
Please enter your batch number(s) to access QC sheets
Certificates of Analysis
Please enter your batch number(s) to access Certificates of Analysis
GC-MS Data
Please enter your batch number(s) to access GC-MS data
References & Background Reading
Background Reading

Lodemann, P., Schorer, G., and Frey, B.M. Wrong molar hemoglobin reference values-a longstanding error that should be corrected. Ann Hematol 89 209 (2010).}

Allen, B.W., Stamler, J.S., and Piantadosi, C.A. Hemoglobin, nitric oxide and molecular mechanisms of hypoxic vasodilation. Trends Mol Med 15(10) 452-460 (2009).}

Schechter, A.N. Hemoglobin research and the origins of molecular medicine. Blood 112(10) 3927-38 (2008).}

Horton, H.R. Structure and function of biomolecules. 3 4.30-4.41 (1993 Jan 1).}

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Technical Support
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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
Contract Research Services

We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research.

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You may be eligible to receive a free sample of Hemoglobin Colorimetric Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA