11β-Hydroxysteroid Dehydrogenase

Cayman Chemical Item Number 10007815

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Description

Source: active recombinant N-terminal His-tagged protein, expressed in E. coli · Mr: 31.4 kDa · 11-β-Hydroxysteroid dehydrogenase (11β-HSD) exists as two isozymes 11β-HSD1 and 11β-HSD2.1 Both isoforms are members of the short-chain dehydrogenase/reductase (SDRs) family.2 11β-HSD1 is a membrane-bound glycoprotein localized on the endoplasmic reticulum. The main catalytic domain of 11β-HSD1 lies within the lumen of the ER. A short five amino acid N-terminal region extends into the cytoplasm and is linked to the catalytic portion through a single transmembrane helix.1 11β-HSD1 is a relatively low affinity NADP-dependent enzyme with a bidirectional catalytic activity. The dehydrogenase reductase activity of 11β-HSD1 catalyzes the conversion of cortisol to cortisone. The primary function of 11β-HSD1 is the oxoreduction of cortisone to the active glucocorticoid cortisol.2 Cortisol is a steroid hormone essential for a variety of physiological and homeostatic functions.

1. Walker, E.A., Clark, A.M., Hewison, M., et al. Functional expression, characterization, and purification of the catalytic domain of human 11-β-hydroxysteroid dehydrogenase type 1. J Biol Chem 276(24) 21343-21350 (2001).

2. Blum, A., Martin, H., and Maser, E. Human 11β-hydroxysteroid dehydrogenase type 1 is enzymatically active in its nonglycosylated form. Biochem Biophys Res Commun 276 428-434 (2000).

Formulation A solution in 50 mM NaPO4, pH 7.6, containing 100 mM sodium chloride and 20% glycerol
Purity ≥95% estimated by SDS-PAGE
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

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Size Price Quantity Subtotal
25 µg $99.00 $0.00
50 µg $188.00 $0.00
100 µg $355.00 $0.00
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Pricing updated 2015-05-30. Prices are subject to change without notice.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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