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Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these 'Thiobarbituric Acid Reactive Substances' (TBARS) is a well-
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1. Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).
2. Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).
Jacob, R.A., Aiello, G.M., Stephensen, C.B., et al. Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. J Nutr 133 740-743 (2003).
Goulart, M., Batoréu, M.C., Rodrigues, A.S., et al. Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 20(5) 311-315 (2005).
Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).
Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).
Wang, L., Tsai, A., and Hsu, P. Substrate binding is the rate-
Scoccia, A.E., Molinuevo, M.S., McCarthy, A.D., et al. A simple method to assess the oxidative susceptibility of low density lipoproteins. BMC Clin Pathol 1 (2001).
Richard, M., Portal, B., Meo, J., et al. Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin Chem 38(5) 704-709 (1992).
Draper, H.H., Squires, E.J., Mahmoodi, H., et al. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15 353-363 (1993).
Ohkawa, H., Ohishi, N., and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95 351-358 (1979).Show all 10 Hide all but first 3
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