TBARS Assay Kit

Cayman Chemical Item Number 10009055

Thiobarbituric Acid Reactive Substances Assay Kit

TBARS Assay Kit

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Description

Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these 'Thiobarbituric Acid Reactive Substances' (TBARS) is a well-established method for screening and monitoring lipid peroxidation.1,2 Modifications of the TBARS assay has been used to evaluate a wide range of samples that include human and animal tissues and fluids, drugs and foods. Even though there remains a controversy cited in literature regarding the specificity of TBARS toward compounds other than MDA, it still remains the most widely employed assay used to determine lipid peroxidation. Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates.

1 Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).

2 Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).

Synonyms
  • Thiobarbituric Acid Reactive Substances Assay Kit
Formal Name
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Jacob, R.A., Aiello, G.M., Stephensen, C.B., et al. Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. J Nutr 133 740-743 (2003).

Goulart, M., Batoréu, M.C., Rodrigues, A.S., et al. Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 20(5) 311-315 (2005).

Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).

Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).

Wang, L., Tsai, A., and Hsu, P. Substrate binding is the rate-limiting step in thromboxane synthase catalysis. J Biol Chem 276(18) 14737-14743 (2001).

Dawn-Linsley, M., Ekinci, F.J., Ortiz, D., et al. Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. J Neurosci Methods 141 219-222 (2005).

Scoccia, A.E., Molinuevo, M.S., McCarthy, A.D., et al. A simple method to assess the oxidative susceptibility of low density lipoproteins. BMC Clin Pathol 1 (2001).

Richard, M., Portal, B., Meo, J., et al. Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin Chem 38(5) 704-709 (1992).

Draper, H.H., Squires, E.J., Mahmoodi, H., et al. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15 353-363 (1993).

Ohkawa, H., Ohishi, N., and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95 351-358 (1979).

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10009055-96WELL
Thiobarbituric Acid 1 ea
TBA Acetic Acid 2 × 1 ea
TBA Sodium Hydroxide 1 ea
TBA Malondialdehyde Standard 1 ea
TBA SDS Solution 1 ea
96-Well Solid Plate (Colorimetric Assay)
96-Well Solid Plate (black)
Size Price Quantity Subtotal
96 wells $185.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-16. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample TBARS Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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