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Cayman is a manufacturer, supplier, and vendor of biochemical reagents, assay kits, forensic chemistry standards, antibodies, and proteins for scientific research. Analytical testing services and custom synthesis are also available.

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2015 Cayman Chemical Company

TBARS Assay Kit

Cayman Chemical Item Number 10009055

Thiobarbituric Acid Reactive Substances Assay Kit

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Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these 'Thiobarbituric Acid Reactive Substances' (TBARS) is a well-established method for screening and monitoring lipid peroxidation.1,2 Modifications of the TBARS assay has been used to evaluate a wide range of samples that include human and animal tissues and fluids, drugs and foods. Even though there remains a controversy cited in literature regarding the specificity of TBARS toward compounds other than MDA, it still remains the most widely employed assay used to determine lipid peroxidation. Cayman’s TBARS Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in plasma, serum, urine, tissue homogenates, and cell lysates.

1. Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).

2. Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).

  • Thiobarbituric Acid Reactive Substances Assay Kit
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Jacob, R.A., Aiello, G.M., Stephensen, C.B., et al. Moderate antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes. J Nutr 133 740-743 (2003).

Goulart, M., Batoréu, M.C., Rodrigues, A.S., et al. Lipoperoxidation products and thiol antioxidants in chromium exposed workers. Mutagenesis 20(5) 311-315 (2005).

Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998).

Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).

Wang, L., Tsai, A., and Hsu, P. Substrate binding is the rate-limiting step in thromboxane synthase catalysis. J Biol Chem 276(18) 14737-14743 (2001).

Dawn-Linsley, M., Ekinci, F.J., Ortiz, D., et al. Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. J Neurosci Methods 141 219-222 (2005).

Scoccia, A.E., Molinuevo, M.S., McCarthy, A.D., et al. A simple method to assess the oxidative susceptibility of low density lipoproteins. BMC Clin Pathol 1 (2001).

Richard, M., Portal, B., Meo, J., et al. Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin Chem 38(5) 704-709 (1992).

Draper, H.H., Squires, E.J., Mahmoodi, H., et al. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15 353-363 (1993).

Ohkawa, H., Ohishi, N., and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95 351-358 (1979).

Show all 10 Hide all but first 3
Thiobarbituric Acid 1 ea
TBA Acetic Acid 2 × 1 ea
TBA Sodium Hydroxide 1 ea
TBA Malondialdehyde Standard 1 ea
TBA SDS Solution 1 ea
96-Well Solid Plate (Colorimetric Assay)
96-Well Solid Plate (black)
Size Price Quantity Subtotal
96 wells $185.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2015-03-05. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample TBARS Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Batch-specific Information

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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