Glucose Colorimetric Assay Kit
Cayman Chemical Item Number 10009582
Glucose, a monosaccharide (or simple sugar), is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body’s cells. Glucose level is tightly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of persistently high (hyperglycemia) or low (hypoglycemia) blood sugar. Diabetes mellitus, characterized by persistent hyperglycemia, is the most prominent disease related to failure of blood sugar regulation. Cayman’s Glucose Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase. The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide. Finally, with horseradish peroxidase as a catalyst, hydrogen peroxide reacts with 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to generate a pink dye with an optimal absorption at 514 nm.
in continental US; may vary elsewhere
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Bernfeld, P. In amylase, α and β. 1 149-158 (1955).
Sugiura, M., and Hirano, K. A new colorimetric method for determination of serum glucose. Clin Chim Acta 75 387-391 (1977).
Slein, M.W. D-glucose, determination with hexokinase and glucose-6-phosphate dehydrrogenase. Methods of Enzymatic Analysis 1 117-123 (1963).
Frost, L.D. Glucose assays revisited: Experimental determination of the glucose concentration in honey. Chem Educ 9(4) 239-241 (2004).
Carroll, J., Smith, N., and Babson, A.L. A colorimetric serum glucose determination using hexokinase and glucose-6-phosphate dehydrogenase. Biochem Med 4 171-180 (1970).
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Pricing updated 2014-04-23.
Prices are subject to change without notice.
To ask for assistance with one of our products please contact a Technical Support Scientist.
Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Glucose Colorimetric Assay Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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