Glucose Colorimetric Assay Kit

Cayman Chemical Item Number 10009582

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Description

Glucose, a monosaccharide (or simple sugar), is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body’s cells. Glucose level is tightly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of persistently high (hyperglycemia) or low (hypoglycemia) blood sugar. Diabetes mellitus, characterized by persistent hyperglycemia, is the most prominent disease related to failure of blood sugar regulation. Cayman’s Glucose Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase. The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide. Finally, with horseradish peroxidase as a catalyst, hydrogen peroxide reacts with 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to generate a pink dye with an optimal absorption at 514 nm.
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Bernfeld, P. In amylase, α and β. 1 149-158 (1955).

Sugiura, M., and Hirano, K. A new colorimetric method for determination of serum glucose. Clin Chim Acta 75 387-391 (1977).

Slein, M.W. D-glucose, determination with hexokinase and glucose-6-phosphate dehydrrogenase. Methods of Enzymatic Analysis 1 117-123 (1963).

Frost, L.D. Glucose assays revisited: Experimental determination of the glucose concentration in honey. Chem Educ 9(4) 239-241 (2004).

Carroll, J., Smith, N., and Babson, A.L. A colorimetric serum glucose determination using hexokinase and glucose-6-phosphate dehydrogenase. Biochem Med 4 171-180 (1970).

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10009582-192WELL
Glucose Assay Standard 1 ea
Glucose Colorimetric Enzyme Mixture 4 × 1 ea
96-Well Solid Plate (Colorimetric Assay)
Sodium Phosphate Assay Buffer 10 mL
Size Price Quantity Subtotal
192 wells $175.00 $0.00
Bulk Contact
Cart Total $0.00

Pricing updated 2015-07-29. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Glucose Colorimetric Assay Kit under the Cayman Challenge program.

Let us analyze your samples for you. See our contract research services.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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