Sphingomyelin Colorimetric Assay Kit

Cayman Chemical Item Number 10009928

SM Assay Kit

Sphingomyelin Colorimetric Assay Kit

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Description

Cayman’s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.1

1 Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Synonyms
  • SM Assay Kit
Formal Name
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Schlitt, A., Hojjati, M.R., von Gizycki, H., et al. Serum sphingomyelin levels are related to the clearance of postprandial remnant-like particles. J Lipid Res 46(2) 196-200 (2005).

Jiang, X., Paultre, F., Pearson, T.A., et al. Plasma sphingomyelin level as a risk factor for coronary artery disease. Arterioscler Thromb Vasc Biol 20(12) 2614-2618 (2000).

Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Schlitt, A., Blankenberg, S., Yan, D., et al. Further evaluation of plasma sphingomyelin levels as a risk factor for coronary artery disease. Nutr Metab 3(5) 1-8 (2006).

He, X., Chen, F., McGovern, M.M., et al. A fluorescence-based, high-throughput sphingomyelin assay for the analysis of Niemann-Pick disease and other disorders of sphingomyelin metabolism. Anal Biochem 306 115-123 (2002).

Testi, R. Sphingomyelin breakdown and cell fate. Trends Biochem Sci 21 468-471 (1996).

Riboni, L., Viani, P., Bassi, R., et al. The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36 153-195 (1997).

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10009928-96WELL
SM Buffer (10X) 1 ea
SM Color Detector 2 × 1 ea
SM Enzyme Mixture 2 × 1 ea
SM Alkaline Phosphatase 1 ea
Sphingomyelinase 1 ea
Sphingomyelin Standard 1 ea
SM Detergent Solution 1 ea
96-Well Solid Plate (Colorimetric Assay)
Size Price Quantity Subtotal
96 wells $254.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Sphingomyelin Colorimetric Assay Kit under the Cayman Challenge program.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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