Sphingomyelin Colorimetric Assay Kit
Cayman Chemical Item Number 10009928
SM Assay Kit
Cayman’s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-
1. Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).
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Riboni, L., Viani, P., Bassi, R., et al. The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36 153-195 (1997).
Testi, R. Sphingomyelin breakdown and cell fate. Trends Biochem Sci 21 468-471 (1996).
He, X., Chen, F., McGovern, M.M., et al. A fluorescence-
Schlitt, A., Blankenberg, S., Yan, D., et al. Further evaluation of plasma sphingomyelin levels as a risk factor for coronary artery disease. Nutr Metab 3(5) 1-8 (2006).
Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).
Jiang, X., Paultre, F., Pearson, T.A., et al. Plasma sphingomyelin level as a risk factor for coronary artery disease. Arterioscler Thromb Vasc Biol 20(12) 2614-2618 (2000).
Schlitt, A., Hojjati, M.R., von Gizycki, H., et al. Serum sphingomyelin levels are related to the clearance of postprandial remnant-
Pricing updated 2015-04-25. Prices are subject to change without notice.
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Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Sphingomyelin Colorimetric Assay Kit under the Cayman Challenge program.
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