Sphingomyelin Colorimetric Assay Kit
Cayman Chemical Item Number 10009928
SM Assay Kit
Cayman’s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-
1. Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).
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Schlitt, A., Hojjati, M.R., von Gizycki, H., et al. Serum sphingomyelin levels are related to the clearance of postprandial remnant-
Jiang, X., Paultre, F., Pearson, T.A., et al. Plasma sphingomyelin level as a risk factor for coronary artery disease. Arterioscler Thromb Vasc Biol 20(12) 2614-2618 (2000).
Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).
Schlitt, A., Blankenberg, S., Yan, D., et al. Further evaluation of plasma sphingomyelin levels as a risk factor for coronary artery disease. Nutr Metab 3(5) 1-8 (2006).
He, X., Chen, F., McGovern, M.M., et al. A fluorescence-
Testi, R. Sphingomyelin breakdown and cell fate. Trends Biochem Sci 21 468-471 (1996).
Riboni, L., Viani, P., Bassi, R., et al. The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36 153-195 (1997).Show all 7 Hide all but first 3
Pricing updated 2015-04-01. Prices are subject to change without notice.
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You may be eligible to receive a free sample Sphingomyelin Colorimetric Assay Kit under the Cayman Challenge program.
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Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.