Sphingomyelin Colorimetric Assay Kit
Item № 10009928
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96 wells $254.00 $0.00

Pricing updated 2015-11-24. Prices are subject to change without notice.

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  • Measure sphingomyelin from 5 to 50 mg/dl
  • Plate-based, colorimetric measurement (585-600 nm)
  • Assay 41 samples in duplicate
  • Measure sphingomyelin in plasma and serum
  • SM Assay Kit

Cayman’s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.1

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Download Kit Booklet Download Safety Data Sheet (SDS)

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Technical Information
  • SM Assay Kit

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere
Stability 1 year
Product Downloads & Resources
Product Downloads

Download Kit Booklet 435 Kb PDF

Download Safety Data Sheet (SDS) 119 Kb PDF

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

QC Sheets
Certificates of Analysis
References & Background Reading
Product Description References

1. Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Background Reading

Riboni, L., Viani, P., Bassi, R., et al. The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36 153-195 (1997).

Testi, R. Sphingomyelin breakdown and cell fate. Trends Biochem Sci 21 468-471 (1996).

He, X., Chen, F., McGovern, M.M., et al. A fluorescence-based, high-throughput sphingomyelin assay for the analysis of Niemann-Pick disease and other disorders of sphingomyelin metabolism. Anal Biochem 306 115-123 (2002).

Schlitt, A., Blankenberg, S., Yan, D., et al. Further evaluation of plasma sphingomyelin levels as a risk factor for coronary artery disease. Nutr Metab 3(5) 1-8 (2006).

Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Jiang, X., Paultre, F., Pearson, T.A., et al. Plasma sphingomyelin level as a risk factor for coronary artery disease. Arterioscler Thromb Vasc Biol 20(12) 2614-2618 (2000).

Schlitt, A., Hojjati, M.R., von Gizycki, H., et al. Serum sphingomyelin levels are related to the clearance of postprandial remnant-like particles. J Lipid Res 46(2) 196-200 (2005).

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Ann Arbor, Michigan 48108 USA


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