WST-8 Cell Proliferation Assay Kit
Item № 10010199
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
96 wells $50.00 $0.00
480 wells $98.00 $0.00
4800 wells $600.00 $0.00

Pricing updated 2015-08-28. Prices are subject to change without notice.

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Description

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.1 Cayman’s WST-8 cell proliferation assay kit provides an easy to use tool for studying the induction or inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.2 Reduction of WST-8 produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. WST-8 is more stable and less cytotoxic than the other tetrazolium salts, making it especially useful for longer incubation periods. The detection sensitivity is higher than that for other tetrazolium salts.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

Download Kit Booklet Download Safety Data Sheet (SDS)

Technical Information

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Stability 1 year
Product Downloads & Resources
Product Downloads

Download Kit Booklet 163 Kb PDF

Download Safety Data Sheet (SDS) 43 Kb PDF

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

Get Batch-Specific Data and Documents by Batch Number

Separate multiple batch numbers with commas

Inserts
QC Sheets
Certificates of Analysis
GCMS Data
References & Background Reading
Product Description References

1. Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

2. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

Background Reading

Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

Technical Support

All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.

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  • Toll Free Phone (USA and Canada Only): (888) 526-5351
  • Direct Phone: (734) 975-3888
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We offer you the deep and diverse knowledge of more than 100 in-house scientists and the expertise that comes from more than three decades of designing and developing the tools to advance biomedical research.

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You may be eligible to receive a free sample of WST-8 Cell Proliferation Assay Kit under the Cayman Challenge program.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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