WST-8 Cell Proliferation Assay Kit

Cayman Chemical Item Number 10010199

WST-8 Cell Proliferation Assay Kit

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Description

Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.1 Cayman’s WST-8 cell proliferation assay kit provides an easy to use tool for studying the induction or inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.2 Reduction of WST-8 produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. WST-8 is more stable and less cytotoxic than the other tetrazolium salts, making it especially useful for longer incubation periods. The detection sensitivity is higher than that for other tetrazolium salts.

1 Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

2 Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Šulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

10010199-4800well
Electron Mediator Solution 50 ea
WST-8 Reagent (powder) 10 × 5 ea
10010199-480WELL
Electron Mediator Solution 5 ea
WST-8 Reagent (powder) 5 ea
10010199-96WELL
Electron Mediator Solution 1 ea
WST-8 Reagent (powder) 1 ea
Size Price Quantity Subtotal
96 wells $50.00 $0.00
480 wells $98.00 $0.00
4800 wells $600.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2014-04-20. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample WST-8 Cell Proliferation Assay Kit under the Cayman Challenge program.

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