WST-8 Cell Proliferation Assay Kit

Cayman Chemical Item Number 10010199

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Defining the mechanisms responsible for alterations in cell cycle progression is crucial to understanding many human diseases, most notably cancer. Cell proliferation assays have been widely used to assess cell cycle regulatory factors such as growth factors, cytokines, mitogens, and drugs.1 Cayman’s WST-8 cell proliferation assay kit provides an easy to use tool for studying the induction or inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of WST-8 by NADH produced in the mitochondria via trans-plasma membrane electron transport and an electron mediator.2 Reduction of WST-8 produces a water-soluble formazan which dissolves directly into the culture medium, eliminating the need for an additional solubilization step. WST-8 is more stable and less cytotoxic than the other tetrazolium salts, making it especially useful for longer incubation periods. The detection sensitivity is higher than that for other tetrazolium salts.

1. Francoeur, A., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3 19-25 (1996).

2. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

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Background Reading

Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11 127-152 (2005).

Šulic, S., Panic, L., Ðikic, I., et al. Deregulation of cell growth and malignant transformation. Croat Med J 46(4) 622-638 (2005).

Electron Mediator Solution 50 ea
WST-8 Reagent (powder) 10 × 5 ea
Electron Mediator Solution 5 ea
WST-8 Reagent (powder) 5 ea
Electron Mediator Solution 1 ea
WST-8 Reagent (powder) 1 ea
Size Price Quantity Subtotal
96 wells $50.00 $0.00
480 wells $98.00 $0.00
4800 wells $600.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2015-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample WST-8 Cell Proliferation Assay Kit under the Cayman Challenge program.

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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA


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