Actin disruption is used to study cell functions in vitro (e.g., migration, endocytosis) and in vivo (e.g., tumor cell invasion). Latrunculin A is a bioactive 2-thiazolidinone macrolide derived from sponges that sequesters G-actin and prevents F-actin assembly. It binds monomeric actin with 1:1 stoichiometry and can be used to block actin polymerization both in vitro (Kd = 0.2 μM)1,2 and in cells (0.5 μM, 30 min).3 Latrunculin A (1-10 μM) causes depolymerization of tumor cell cytoskeleton within ten minutes.4 Overnight treatment of cells with latrunculin A (10 μM) strongly suppresses actin synthesis.5 Prolonged cell treatment blocks dexamethasone-induced changes in actin cytoskeleton with no effect on cell viability.6
1Coué, M., Brenner, S.L., Spector, I., et al. Inhibition of actin polymerization by latrunculin A. FEBS Lett213(2)316-318(1987).
2Yarmola, E.G., Somasundaram, T., Boring, T.A., et al. Actin-latrunculin A structure and function. J Biol Chem275(36)28120-28127(2000).
3Loubéry, S., Wilhelm, C., Hurbain, I., et al. Different microtubule motors move early and late endocytic compartments. Traffic9492-509(2008).
4Hayot, C., Debeir, O., Van Ham, P., et al. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicol Appl Pharmacol21130-40(2006).
5Lyubimova, A., Bershadsky, A.D., and Ben-Ze'ev, A.Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. J Cell Biochem761-12(1999).
6Liu, X., Wu, Z., Sheibani, N., et al. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Exp Eye Res77181-188(2003).
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Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.
Manufacturer, supplier, and vendor of biochemical reagents, assay kits, forensic chemistry standards, antibodies, and proteins for scientific research
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