Glucokinase (human pancreatic recombinant)

Cayman Chemical Item Number 10989

ATP:D-Hexose 6-Phosphotransferase GCK; Hexokinase D; Human Hexokinase IV isoform 1

Glucokinase (human pancreatic recombinant)

See more

Description

Source: active recombinant C-terminal FLAG-tagged protein expressed in E. coli · Mr: 52.8 kDa · Glucokinase (GCK) is an enzyme that catalyses the ATP-dependent phosphorylation of glucose to produce glucose-6-phosphate. This is the first and rate-limiting step in the glycolytic pathway. GCK was initially described in liver but is present in other tissues as well, such as pancreatic B cells, neurons, the pituitary, and endocrine cells of the gut.1 Unlike other members of the hexokinase family (human hexokinases I-III), GCK has a high S0.5 value for glucose, the concentration giving half maximal activity. The S0.5 value of GCK for glucose is 5-8 mM, which is near the normal fasting blood glucose levels of 3.9-5.5 mM. In contrast, hexokinases I-III have S0.5 values for glucose in the range of 20-130 µM.2 The distribution of GCK-expressing cells and its sensitivity to glucose is believed to play a central role in maintaining glucose homeostasis. Mutations of the glucokinase gene are associated with non-insulin-dependent (type 2) diabetes mellitus (NIDDM).3 The kinetic defects associated with glucokinase mutations include reduced catalytic activity, increased KM for glucose, and/or increased KM for ATP.4 GCK activity from beta cells of the pancreatic islets is responsible for regulating insulin release while GCK from pancreatic alpha cells controls the release of glucagon.5

1 Massa, M.L., Gagliardino, J.J., and Francini, F. Liver glucokinase: An overview on the regulatory mechanisms of its activity. Life 63(1) 1-6 (2011).

2 Mahalingam, B., Cuesta-Munoz, A., Davis, E.A., et al. Structural model of human glucokinase in complex with glucose and ATP. Diabetes 48 1698-1705 (1999).

3 Tahrani, A.A., Bailey, C.J., Del Prato, S., et al. Management of type 2 diabetes: New and future developements in treatment. New Horizons 6736(11) 60207-60209 (2011).

4 Gidh-Jain, M., Takeda, J., Xu, L.Z., et al. Glucokinase mutations associated with non-insulin-dependent (type 2) diabetes mellitus have decreased enzymatic activity: Implications for structure/function relationships. Proc Natl Acad Sci USA 90 1932-1936 (1993).

5 Matschinsky, F.M. Regulation of pancreatic b-cell glucokinase. Diabetes 51(Suppl. 3) S394-S404 (2002).

Synonyms
  • ATP:D-Hexose 6-Phosphotransferase GCK
  • Hexokinase D
  • Human Hexokinase IV isoform 1
Formal Name
Formulation 50 mM potassium phosphate, pH 7.4, containing 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 10% glycerol
Purity ≥90%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Download free InCHI Key generation software

Size Price Quantity Subtotal
5 µg $40.00 $0.00
10 µg $76.00 $0.00
25 µg $180.00 $0.00
Bulk Contact
Cart Total $0.00

Please Login or Register to use this page.

Pricing updated 2014-04-19. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

Glucokinase (human liver recombinant)
Glucose Colorimetric Assay Kit

Downloads

Batch-specific Information

Get batch-specific data and documents by batch number


Separate multiple batch numbers with commas

Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · Experimental Biology 2014 · San Diego, California · April 26-30, 2014 · Booth 323
Other Resources
About Cayman Management Team Company Profile ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map Conference Travel Grants

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2014 Cayman Chemical Company

Manufacturer, supplier, and vendor of biochemical reagents, assay kits, forensic chemistry standards, antibodies, and proteins for scientific research · Buy now! · Analytical testing services and custom synthesis also available

Lost password?