Glucokinase (human pancreatic recombinant)
Item № 10989
Purity ≥90%
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SIZE PRICE QUANTITY SUBTOTAL
CART TOTAL $0.00
5 µg $40.00 $0.00
10 µg $76.00 $0.00
25 µg $180.00 $0.00

Pricing updated 2015-09-04. Prices are subject to change without notice.

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Description
Synonyms
  • ATP:D-Hexose 6-Phosphotransferase GCK
  • Hexokinase D
  • Human Hexokinase IV isoform 1

Glucokinase (GCK) is an enzyme that catalyses the ATP-dependent phosphorylation of glucose to produce glucose-6-phosphate. This is the first and rate-limiting step in the glycolytic pathway. GCK was initially described in liver but is present in other tissues as well, such as pancreatic B cells, neurons, the pituitary, and endocrine cells of the gut.1 Unlike other members of the hexokinase family (human hexokinases I-III), GCK has a high S0.5 value for glucose, the concentration giving half maximal activity. The S0.5 value of GCK for glucose is 5-8 mM, which is near the normal fasting blood glucose levels of 3.9-5.5 mM. In contrast, hexokinases I-III have S0.5 values for glucose in the range of 20-130 µM.2 The distribution of GCK-expressing cells and its sensitivity to glucose is believed to play a central role in maintaining glucose homeostasis. Mutations of the glucokinase gene are associated with non-insulin-dependent (type 2) diabetes mellitus (NIDDM).3 The kinetic defects associated with glucokinase mutations include reduced catalytic activity, increased KM for glucose, and/or increased KM for ATP.4 GCK activity from beta cells of the pancreatic islets is responsible for regulating insulin release while GCK from pancreatic alpha cells controls the release of glucagon.5

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Technical Information
Synonyms
  • ATP:D-Hexose 6-Phosphotransferase GCK
  • Hexokinase D
  • Human Hexokinase IV isoform 1
Purity ≥90%
Source active recombinant C-terminal FLAG-tagged protein expressed in E. coli
MW 52.8 kDa
Formulation 50 mM potassium phosphate, pH 7.4, containing 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 10% glycerol

WARNING - This product is not for human or veterinary use.

Shipping & Storage
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Stability 6 months
Product Downloads & Resources
Product Downloads

Download Product Insert 114 Kb PDF

Download Safety Data Sheet (SDS) 26 Kb PDF

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Additional Information

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Inserts
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GCMS Data
References & Background Reading
Product Description References

1. Massa, M.L., Gagliardino, J.J., and Francini, F. Liver glucokinase: An overview on the regulatory mechanisms of its activity. Life 63(1) 1-6 (2011).

2. Mahalingam, B., Cuesta-Munoz, A., Davis, E.A., et al. Structural model of human glucokinase in complex with glucose and ATP. Diabetes 48 1698-1705 (1999).

3. Tahrani, A.A., Bailey, C.J., Del Prato, S., et al. Management of type 2 diabetes: New and future developements in treatment. New Horizons 6736(11) 60207-60209 (2011).

4. Gidh-Jain, M., Takeda, J., Xu, L.Z., et al. Glucokinase mutations associated with non-insulin-dependent (type 2) diabetes mellitus have decreased enzymatic activity: Implications for structure/function relationships. Proc Natl Acad Sci USA 90 1932-1936 (1993).

5. Matschinsky, F.M. Regulation of pancreatic b-cell glucokinase. Diabetes 51(Suppl. 3) S394-S404 (2002).

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