Nitrotyrosine Affinity Sorbent

Cayman Chemical Item Number 389549

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Description

The Nitrotyrosine Affinity Sorbent is designed for IP of nitrated proteins from biological samples. This is an effective way to concentrate nitrated proteins for subsequent detection by a different anti-nitrotyrosine antibody, such as Cayman’s nitrotyrosine polyclonal antibody (Catalog No. 189540), by WB analysis. Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide (6.7 x 109 M−1sec−1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo.1,2 Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including artherosclerotic lesions of human coronary arteries, postischemic heart, and placenta during preeclampsia.3,4,5 Increased nitration of proteins in motor neurons has been identified in patients with ALS (amyotrophic lateral sclerosis) and may be due to mutations in superoxide dismutase.2,6,7,8

1. Beckman, J.S., Chen, J., Ischiropoulos, H., et al. Oxidative chemistry of peroxynitrite. Methods Enzymol 233 229-240 (1994).

2. Beckman, J.S., and Koppenol, W.H. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly. Am J Physiol 271 C1424-C1437 (1996).

3. Beckman, J.S., Ye, Y.Z., Anderson, P.G., et al. Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry. Biol Chem Hoppe-Seyler 375 81-88 (1994).

4. Wang, P., and Zweier, J.L. Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury. J Biol Chem 271 29223-29230 (1996).

5. Myatt, L., Rosenfield, R.B., Eis, A.L.W., et al. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 28 488-493 (1996).

6. Abe, K., Pan, L., Watanabe, M., et al. Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis. Neurol Res 19 124-128 (1997).

7. Chou, S.M., Wang, H.S., and Komai, K. Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: An immunohistochemical study. J Chem Neuroanat 10 249-258 (1996).

8. Ferrante, R.J., Shinobu, L.A., Schulz, J.B., et al. Increased 3-nitrotyrosine and oxidative damage in mice with a human copper/zinc superoxide dismutase mutation. Ann Neurol 42 326-334 (1997).

Formulation 200 µg of IgG per 200 µl of agarose in 400 µl of TBS, pH 7.4, containing 0.02% sodium azide
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

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Size Price Quantity Subtotal
200 µg $259.00 $0.00
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Cart Total $0.00

Pricing updated 2015-07-02. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

Nitrotyrosine
Nitrotyrosine BSA
Nitrotyrosine Polyclonal Antibody
Peroxynitrite
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Cayman Chemical Company regularly evaluates our shipping methods to ensure product stability is maintained and our corporate environmental impact is minimized. Products selected for review are evaluated and shipping methods are certified by our Scientists. While occasionally our method of shipping a product may change, rest assured the shipping conditions on all Cayman Chemical products have been reviewed and validated.

Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

Cayman Chemical

1180 East Ellsworth Road

Ann Arbor, Michigan 48108 USA

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