Prostaglandin E2 Express EIA Kit

Cayman Chemical Item Number 500141

PGE2 Express ELISA Kit

Prostaglandin E2 Express EIA Kit

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Description

Limit of detection: 80% B/B0: 36 pg/ml · Sensitivity: 50% B/B0: 125 pg/ml · PGE2 is one of the primary COX products of arachidonic acid and one of the most widely investigated prostaglandins. Its activity influences inflammation, fertility and parturition, gastric mucosal integrity, and immune modulation.1,2,3,4 Because of its involvement in inflammation, PGE2 is the key indicator of non-steroidal anti-inflammatory drugs efficacy via inhibition of COX-1 and COX-2. Cayman Chemical’s PGE2 Express EIA is a competitive assay that permits the rapid measurement of PGE2 from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE2 monoclonal, the same used in our PGE2 Monoclonal EIA kit (Catalog No. 514010). While Catalog No. 514010 offers superior sensitivity (IC50 < 50 pg/ml), the new PGE2 Express Assay offers the convenience of a fast assay while still achieving an IC50 of 125 pg/ml and a detection limit (80% B/B0) of approximately 35 pg/ml.

1 Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E2 in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

2 Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E2 gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

3 Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE2, 15-methyl PGE2, and 16,16-dimethyl PGE2. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

4 Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E2 down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

Synonyms
  • PGE2 Express ELISA Kit
Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Prostaglandin E2 100%
Prostaglandin E2 Ethanolamide 100%
Prostaglandin E2-1-glyceryl ester 100%
Prostaglandin E3 43.0%
Prostaglandin E1 18.7%
8-iso Prostaglandin E2 2.5%
Sulprostone 1.3%
6-keto Prostaglandin F1α 1.0%
8-iso Prostaglandin F2α 0.25%
Prostaglandin A2 0.04%
13,14-dihydro-15-keto Prostaglandin E2 <0.01%
Arachidonic acid <0.01%
Arachidonoyl Ethanolamide <0.01%
O-Arachidonoyl Ethanolamide <0.01%
Butaprost <0.01%
Conjugated Linoleic Acid (10E,12Z) <0.01%
8(S),15(S)-DiHETE <0.01%
Leukotriene B4 <0.01%
Misoprostol <0.01%
Misoprostol (free acid) <0.01%
tetranor-PGEM <0.01%
tetranor-PGFM <0.01%
Prostaglandin A1 <0.01%
8-iso Prostaglandin A2 <0.01%
Prostaglandin A3 <0.01%
Prostaglandin B1 <0.01%
Prostaglandin B2 <0.01%
Prostaglandin D2 <0.01%
11-deoxy Prostaglandin E2 <0.01%
16,16-dimethyl Prostaglandin E2 <0.01%
19(R)-hydroxy Prostaglandin E2 <0.01%
20-hydroxy Prostaglandin E2 <0.01%
15-keto Prostaglandin E2 <0.01%
Prostaglandin F1α <0.01%
Prostaglandin F2α <0.01%
13,14-dihydro-15-keto Prostaglandin F2α <0.01%
Prostaglandin F3α <0.01%
Prostaglandin J2 <0.01%
15-deoxy-Δ12,14-Prostaglandin J2 <0.01%
Thromboxane B2 <0.01%
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Background Reading

Hamberg, M., and Samuelsson, B. On the metabolism of prostaglandins E1 and E2 in man. J Biol Chem 246 6713-6721 (1971).

Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE2. Prostaglandins 19 917-931 (1980).

Robert, A., Schultz, J.R., Nezamis, J.E., et al. Gastric antisecretory and antiulcer properties of PGE2, 15-methyl PGE2, and 16,16-dimethyl PGE2. Intravenous, oral and intrajejunal administration. Gastroenterology 70 359-370 (1976).

Antoine, C., Lellouche, J., Maclouf, J., et al. Development of enzyme immunoassays for leukotrienes using acetylcholinesterase. Biochim Biophys Acta 1075 162-168 (1991).

Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).

Jackson, G.M., Sharp, H.T., and Varner, M.W. Cervical ripening before induction of labor: A randomized trial of prostaglandin E2 gel versus low-dose oxytocin. Am J Obstet Gynecol 171 1092-1096 (1994).

Oudot, F., Grynberg, A., and Sergiel, J.P. Eicosanoid synthesis in cardiomyocytes: Influence of hypoxia, reoxygenation, and polyunsaturated fatty acids. Am J Physiol 268 H308-H315 (1995).

Zhang, Y., Shaffer, A., Portanova, J., et al. Inhibition of cyclooxygenase-2 rapidly reverses inflammatory hyperalgesia and prostaglandin E2 production. J Pharmacol Exp Ther 283 1069-1075 (1997).

Taniguchi, Y., Yokoyama, K., Inui, K., et al. Inhibition of brain cyclooxygenase-2 activity and the antipyretic action of nimesulide. Eur J Pharmacol 330 221-229 (1997).

Kuwata, H., Nakatani, Y., Murakami, M., et al. Cytosolic phospholipase A2 is required for cytokine-induced expression of type IIA secretory phospholipase A2 that mediates optimal cyclooxygenase-2-dependent delayed prostaglandin E2 generation in rat 3Y1 fibroblasts. J Biol Chem 273 1733-1740 (1998).

Marshall, L.A., Bolognese, B., Winkler, J.D., et al. Depletion of human monocyte 85-kDa phospholipase A2 does not alter leukotriene formation. J Biol Chem 272 759-765 (1997).

Thivierge, M., and Rola-Pleszczynski, M. Up-regulation of inducible cyclooxygenase gene expression by platelet-activating factor in activated rat alveolar macrophages. J Immunol 154 6593-6599 (1995).

Portanova, J.P., Zhang, Y., Anderson, G.D., et al. Selective neutralization of prostaglandin E2 blocks inflammation, hyperalgesia, and interleukin 6 production in vivo. J Exp Med 184 883-891 (1996).

Ebisawa, M., Umemiya, H., Ohta, K., et al. Retinoid X receptor-antagonistic diazepinylbenzoic acids. Chem Pharm Bull 47(12) 1778-1786 (1999).

Muth-Selbach, U.S., Tegeder, I., Brune, K., et al. Acetaminophen inhibits spinal prostaglandin E2 release after peripheral noxious stimulation. Anesthesiology 91 231-239 (1999).

Newberry, R.D., Stenson, W.F., and Lorenz, R.G. Cyclooxygenase-2-dependent arachidonic acid metabolites are essential modulators of the intestinal immune response to dietary antigen. Nat Med 5 900-906 (1999).

Johnson, R.D., Polakoski, K., Everson, W.V., et al. Aspirin induces increased expression of both prostaglandin H synthase-1 and prostaglandin H synthase-2 in cultured human placental trophoblast. Am J Obstet Gynecol 177 78-85 (1997).

Pradelles, P., Grassi, J., and Maclouf, J.A. Enzyme immunoassays of eicosanoids using acetylcholinesterase as label: An alternative to radioimmunoassay. Anal Chem 57 1170-1173 (1985).

Willis, A.L., and Cornelsen, M. Repeated injection of prostaglandin E2 in rat paws induces chronic swelling and a marked decrease in pain threshold. Prostaglandins 3 353-357 (1973).

Arvind, P., Papavassiliou, E.D., Tsioulias, G.J., et al. Prostaglandin E2 down-regulates the expression of HLA-DR antigen in human colon adenocarcinoma cell lines. Biochemistry 34 5604-5609 (1995).

Thibodeaux, J.K., Broussard, J.R., Godke, R.A., et al. Stimulation of progesterone production in bovine luteal cells by co-incubation with bovine blastocyst-stage embryos or trophoblastic vesicles. J Reprod Fertil 101 657-662 (1994).

Roshak, A.K., Jackson, J.R., McGough, K., et al. Manipulation of distinct NFκB proteins alters interleukin-1β-induced human rheumatoid synovial fibroblast prostaglandin E2 formation. J Biol Chem 271 31496-31501 (1996).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Wilborn, J., Crofford, L.J., Burdick, M.D., et al. Cultured lung fibroblasts isolated from patients with idiopathic pulmonary fibrosis have a diminished capacity to synthesize prostaglandin E2 and to express cyclooxygenase-2. J Clin Invest 95 1861-1868 (1995).

Swinney, D.C., Mak, A.Y., Barnett, J., et al. Differential allosteric regulation of prostaglandin H synthase 1 and 2 by arachidonic acid. J Biol Chem 272 12393-12398 (1997).

Murakami, M., Kuwata, H., Amakasu, Y., et al. Prostaglandin E2 amplifies cytosolic phospholipase A2- and cyclooxygenase-2-dependent delayed prostaglandin E2 generation in mouse osteoblastic cells. Enhancement by secretory phospholipase A2. J Biol Chem 272 19891-19897 (1997).

Viganò, T., Habib, A., Hernandez, A., et al. Cyclooxygenase-2 and synthesis of PGE2 in human bronchial smooth-muscle cells. Am J Respir Crit Care Med 155 864-868 (1997).

Mirzoeva, O.K., and Calder, P.C. The effect of propolis and its components on eicosanoid production during the inflammatory response. Prostaglandins Leukot Essent Fatty Acids 55 441-449 (1996).

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500141-96WELL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin E2 Express AChE Tracer 100 dtn
Prostaglandin E2 Express EIA Monoclonal Antibody 100 dtn
Prostaglandin E2 Express EIA Standard 1 ea
500141-96SOLID
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Prostaglandin E2 Express AChE Tracer 100 dtn
Prostaglandin E2 Express EIA Monoclonal Antibody 100 dtn
Prostaglandin E2 Express EIA Standard 1 ea
500141-480WELL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin E2 Express AChE Tracer 500 dtn
Prostaglandin E2 Express EIA Monoclonal Antibody 500 dtn
Prostaglandin E2 Express EIA Standard 1 ea
500141-480SOLID
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Prostaglandin E2 Express AChE Tracer 500 dtn
Prostaglandin E2 Express EIA Monoclonal Antibody 500 dtn
Prostaglandin E2 Express EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $285.00 $0.00
96 strip wells $285.00 $0.00
480 solid wells $1,069.00 $0.00
480 strip wells $1,069.00 $0.00
Bulk Contact
Cart Total $0.00

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  • All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2014-04-16. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin E2 Express EIA Kit under the Cayman Challenge program.

Is this kit right for my sample type? Prostaglandin E2 Recommendation Chart

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Cayman Chemical was formed over thirty years ago by demonstrating the value of naturally growing gorgonian corals as a renewable, economically viable source of prostaglandins. Today we operate our business knowing that we owe our start to the renewable resources our environment had provided. As Cayman Chemical continues to grow we consistently evaluate our business processes to ensure we are operating at the highest environmental standards.

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