Cortisol EIA Kit

Cayman Chemical Item Number 500360

Cortisol ELISA Kit

Cortisol EIA Kit

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Description

Limit of detection: 80% B/B0: 35 pg/ml · Sensitivity: 50% B/B0: 180 pg/ml · Cortisol is a glucocorticoid produced by the adrenal cortex in response to adrenocorticotropic hormone (ACTH). It is secreted with a circadian periodicity, and peaks just prior to waking in the morning. Cortisol is often elevated in major depressive disorder, certain forms of hypertension, stress, AIDS, and in the visceral fat of obese individuals. Cortisol can be measured in many matrices including blood, urine, and saliva. In serum, approximately 90-95% of cortisol is bound to proteins. Urinary cortisol is not bound to proteins, but its levels are dependent on glomerular and tubular function. In saliva, approximately 67% of cortisol is unbound. There is generally good correlation between cortisol measurements in saliva and serum. Cayman’s Cortisol EIA Kit is a competitive assay that can be used for quantification of cortisol in urine, plasma, and other sample matrices. The EIA typically displays an IC50 (50% B/B0) of aproximately 180 pg/ml and a detection limit (80% B/B0) of approximately 35 pg/ml.
Synonyms
  • Cortisol ELISA Kit
Formal Name
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Cortisol 100%
Prednisolone 4.0%
Cortexolone 1.6%
11-Deoxycorticosterone 0.23%
17-Hydroxyprogesterone 0.23%
Cortisol Glucuronide 0.15%
Corticosterone 0.14%
Cortisone 0.13%
Androstenedione <0.01%
Enterolactone <0.01%
Estrone <0.01%
17-Hydroxypregnenolone <0.01%
Pregnenolone <0.01%
Testosterone <0.01%
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Background Reading

Masuzaki, H., Paterson, J., Shinyama, H., et al. A transgenic model of visceral obesity and the metabolic syndrome. Science 294 2166-2170 (2001).

Maclouf, J., Grassi, J., and Pradelles, P. Development of enzyme-immunoassay techniques for the measurement of eicosanoids. 355-364 (1987).

Pradelles, P., Grassi, J., and Maclouf, J. Enzyme immunoassays of eicosanoids using acetylcholinesterase. Methods Enzymol 187 24-34 (1990).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Krieger, D.T. Rhythms of ACTH and corticosteroid secretion in health and disease, and their experimental modification. J Steroid Biochem 6 785-791 (1975).

Rask, E., Olsson, T., Söderberg, S., et al. Tissue-specific dysregulation of cortisol metabolism in human obesity. J Clin Endocrinol Metab 86(3) 1418-1421 (2001).

Stulnig, T.M., and Waldhäusl, W. 11β-Hydroxysteroid dehydrogenase type 1 in obesity and type 2 diabetes. Diabetologia 47 1-11 (2004).

Aardal, E., and Holm, A. Cortisol in saliva-reference ranges and relation to cortisol in serum. Eur J Clin Chem Clin Biochem 33 927-932 (1995).

Quinkler, M., and Stewart, P.M. Hypertension and the cortisol-cortisone shuttle. J Clin Endocrinol Metab 88(6) 2384-2392 (2003).

Christeff, N., Michon, C., Goertz, G., et al. Abnormal free fatty acids and cortisol concentrations in the serum of AIDS patients. Eur J Cancer Clin Oncol 24(7) 1179-1183 (1988).

Brown, E.S., Varghese, F.P., and McEwen, B.S. Association of depression with medical illness: Does cortisol play a role? Biol Psychiatry 55 1-9 (2004).

Varghese, F.P., and Brown, E.S. The hypothalamic-pituitary-adrenal axis in major depressive disorder: A brief primer for primary care physicians. Prim Care Companion J Clin Psychiatry 3 151-155 (2001).

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500360-96SOLID
Cortisol AChE Tracer 100 dtn
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Blue EIA Kit Box (96 Well)
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cortisol EIA Monoclonal Antibody 100 dtn
Cortisol EIA Standard 1 ea
500360-480WELL
Cortisol AChE Tracer 500 dtn
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Green EIA Kit Box (480 Well)
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cortisol EIA Monoclonal Antibody dtn
Cortisol EIA Standard 1 ea
500360-96WELL
Cortisol AChE Tracer 100 dtn
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Blue EIA Kit Box (96 Well)
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Cortisol EIA Monoclonal Antibody 100 dtn
Cortisol EIA Standard 1 ea
500360-480SOLID
Cortisol AChE Tracer 500 dtn
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
EIA Tracer Dye 1 ea
EIA Antiserum Dye 1 ea
Green EIA Kit Box (480 Well)
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Cortisol EIA Monoclonal Antibody 500 dtn
Cortisol EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $270.00 $0.00
96 strip wells $270.00 $0.00
480 solid wells $1,013.00 $0.00
480 strip wells $1,013.00 $0.00
Bulk Contact
Cart Total $0.00

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  • All 480 well kits are supplied with a single 500 dtn vial of antiserum and tracer, or Fab' conjugate that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2014-04-18. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cortisol EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Upon receipt, it is important to check the storage requirements of your products and place them at the appropriate temperature. Products shipped without ice may require frozen storage for long term stability.

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